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Alexa Fluor® 488 anti-mouse/human Mac-2 (Galectin-3) Antibody

Pricing & Availability
Clone
M3/38 (See other available formats)
Regulatory Status
RUO
Other Names
Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype
Rat IgG2a, κ
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Product Citations
publications
M3slash38_AF488_1_051311
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with CD107b Alexa Fluor® 647 and Mac-2 (clone M3/38) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • M3slash38_AF488_1_051311
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with CD107b Alexa Fluor® 647 and Mac-2 (clone M3/38) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • M3slash38_AF488_2_051311
Compare all formats See Alexa Fluor® 488 spectral data
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125410 100 µg £182
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Description

Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against galectin-3 of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-37.

Application References

(PubMed link indicates BioLegend citation)
  1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
  2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
  3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
  4. Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
  5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
  6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
  7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
  8. Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
  9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
  10. Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed
Product Citations
  1. Aylan B, et al. 2023. Nat Microbiol. 8:803. PubMed
  2. Bussi C, et al. 2022. Nat Commun. 13:7338. PubMed
  3. Blackburn CMR, et al. 2021. Atherosclerosis. 330:76. PubMed
  4. Bussi C, et al. 2018. J Cell Sci. 131. PubMed
  5. Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed
RRID
AB_10730886 (BioLegend Cat. No. 125410)

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.

Gene ID
16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org
Go To Top Version: 4    Revision Date: 11/05/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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