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Alexa Fluor® 488 anti-mouse I-Ab (Aβb) Antibody

Pricing & Availability
Clone
25-9-17 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Mouse (C3H) IgG2a, κ
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Product Citations
publications
25-9-17_Alx488_122607
C57BL/6 mouse splenocytes stained with 25-9-17 Alexa Fluor® 488
  • 25-9-17_Alx488_122607
    C57BL/6 mouse splenocytes stained with 25-9-17 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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114407 25 µg £70
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Description

The 25-9-17 antibody reacts with the beta chain of the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2d, p, q haplotypes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6.

Application References

(PubMed link indicates BioLegend citation)
  1. Wall KA, et al. 1983. J. Immunol. 131:1056. (IP)
  2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747.
  3. Ozato K, et al. 1981. J. Immunol. 126:317.
  4. Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC)
  5. Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC)
  6. Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed
  7. Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed.
  8. Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed.
RRID
AB_493144 (BioLegend Cat. No. 114407)

Antigen Details

Structure
ß chain of I-Ab MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ab on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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