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Alexa Fluor® 488 anti-mouse IgE Antibody

Pricing & Availability
Clone
RME-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin E
Isotype
Rat IgG1, κ
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Product Citations
publications
RME-1_AF488_IgE_Antibody_FC_1_041714
C57BL/6 mouse bone marrow cells were stained with CD45 PE, Ly-6G PerCP and IgE (clone RME-1) Alexa Fluor® 488 (top image) or rat IgG1, κ Alexa Fluor® 488 isotype control (bottom image). Data was gated on CD45 positive cells.
  • RME-1_AF488_IgE_Antibody_FC_1_041714
    C57BL/6 mouse bone marrow cells were stained with CD45 PE, Ly-6G PerCP and IgE (clone RME-1) Alexa Fluor® 488 (top image) or rat IgG1, κ Alexa Fluor® 488 isotype control (bottom image). Data was gated on CD45 positive cells.
  • RME-1_AF488_IgE_Antibody_FC_2_041714
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
406909 25 µg £61
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406910 100 µg £157
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Description

The RME-1 monoclonal antibody reacts with immunoglobulin E (IgE) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RME-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail, including IgE
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone also recognizes IgE when bound to FceRI.

Application References

(PubMed link indicates BioLegend citation)
  1. Jeong KT, et al. 2012. Int Immunopharmacol. 13:377. PubMed
Product Citations
  1. Dos Santos M, et al. 2023. Nat Commun. 4333:14. PubMed
RRID
AB_2563455 (BioLegend Cat. No. 406909)
AB_2563455 (BioLegend Cat. No. 406910)

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Go To Top Version: 3    Revision Date: 01/26/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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