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Alexa Fluor® 488 anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
8.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Syrian Hamster IgG
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Product Citations
publications
8_1_1_A488_022309.jpg
TE-71 mouse thymic epithelial stromal cell line stained with 8.1.1 Alexa Fluor® 488
  • 8_1_1_A488_022309.jpg
    TE-71 mouse thymic epithelial stromal cell line stained with 8.1.1 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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127405 25 µg £105
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127406 100 µg £221
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
  2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
  3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
  4. Algars A, et al. 2011. Blood 117:4387. PubMed
  5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
  6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
  7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  2. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  3. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  4. Hoorweg K, et al. 2015. J Immunol. 195: 4257 - 4263. PubMed
  5. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  6. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  7. Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed
  8. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  9. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  10. Nadafi R, et al. 2020. Cell Reports. 30(12):4110-4123. PubMed
  11. Jung M, et al. 2016. Sci Signal. 9: ra64. PubMed
  12. Durré T, et al. 2018. Nat Commun. 9:5178. PubMed
RRID
AB_2161930 (BioLegend Cat. No. 127405)
AB_2161930 (BioLegend Cat. No. 127406)

Antigen Details

Structure
43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Cell Type
Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.

Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org
Go To Top Version: 3    Revision Date: 02/03/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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