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Alexa Fluor® 488 anti-Syntaxin Antibody

Pricing & Availability
Clone
SP8 (See other available formats)
Regulatory Status
RUO
Other Names
Syntaxin-1A, neuron-specific antigen HPC-1
Isotype
Mouse IgG1
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Product Citations
publications
SP8_A488_Syntaxin_Antibody_1_121219.png
IHC staining of Alexa Fluor® 488 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm
  • SP8_A488_Syntaxin_Antibody_1_121219.png
    IHC staining of Alexa Fluor® 488 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm
  • SP8_A488_Syntaxin_Antibody_2_121219.png
    IHC staining of Alexa Fluor® 488 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm
Compare all formats See Alexa Fluor® 488 spectral data
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827009 25 µg £109
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827010 100 µg £221
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Description

Syntaxin, also known as syntaxin-1A and neuron-specific antigen HPC-1, is part of the Q-SNARE protein family that participates in exocytosis. Syntaxins have a SNARE domain, an N-terminal regulatory domain, and a single C-terminal transmembrane domain. Syntaxin drives the spontaneous calcium-independent fusion of synaptic vesicles that contain v-SNARE proteins, and may form part of the fusion pore of exocytosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a crude synaptic preparation from a post-mortem human brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunohistochemistry (IHC-P) and western blotting (WB).

Additional Product Notes

The reactivity of this conjugate is weak in human tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Honer WG, et al. 1997. Neuroscience 78(1):99-110.
  2. Honer WG, et al. 1993. Brain Res. 609(1-2):9-20.
RRID
AB_2820131 (BioLegend Cat. No. 827009)
AB_2820131 (BioLegend Cat. No. 827010)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6804 View all products for this Gene ID
UniProt
View information about Syntaxin on UniProt.org
Go To Top Version: 1    Revision Date: 12/12/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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