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Alexa Fluor® 488 anti-TMEM119 (Extracellular) Antibody

Pricing & Availability
Clone
A16075D (See other available formats)
Regulatory Status
RUO
Other Names
Transmembrane protein 119
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A. A16075D_A-488_-TMEM119_ICFC_070924
CHO cells untransfected (negative control, open histogram), or transiently transfected with a human TMEM119 expression vector (positive control, two peaks are observed which represent cells which have not or have successfully incorporated the TMEM119 expression vector, filled histogram). Samples were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 488 anti-TMEM119 (clone A16075D) or Alexa Fluor® 488 Mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for non-transfected or transfected cells) (Cat. No. 400329).
  • A. A16075D_A-488_-TMEM119_ICFC_070924
    CHO cells untransfected (negative control, open histogram), or transiently transfected with a human TMEM119 expression vector (positive control, two peaks are observed which represent cells which have not or have successfully incorporated the TMEM119 expression vector, filled histogram). Samples were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 488 anti-TMEM119 (clone A16075D) or Alexa Fluor® 488 Mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for non-transfected or transfected cells) (Cat. No. 400329).
  • b. A16075D_A-488_-TMEM119_ICC_070924
    Non-transfected CHO cells (negative control, panel A) or cells transiently transfected with a human TMEM119 expression vector (positive control, panel B) were fixed with PFA Fixation Buffer (Cat. No. 420801), permeabilized with methanol, and blocked with 5% FBS. Cells were stained with Alexa Flour® 488 anti-TMEM119 (clone A16075D). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured in a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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853317 25 µg £124
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853318 100 µg £291
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Description

Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human TMEM119 protein (extracellular domain)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron.

*The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.

Application References

(PubMed link indicates BioLegend citation)

  1. Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed

RRID
AB_3662340 (BioLegend Cat. No. 853317)
AB_3662340 (BioLegend Cat. No. 853318)

Antigen Details

Structure
TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
Distribution

Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues.
Cellular distribution: Membrane and endoplasmic reticulum.

Function
TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
Interaction
TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113
  2. Satoh J, et al. 2016. Neuropathology. 36:39-49
  3. Kawao N and Kaji H. 2015. J Cell Biochem. 116:687
  4. Zrzavy T, et al. 2017. Brain. 140:1900-1913
Gene ID
338773 View all products for this Gene ID
UniProt
View information about TMEM119 on UniProt.org
Go To Top Version: 1    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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