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Alexa Fluor® 594 anti-eIF4E Antibody

Pricing & Availability
Clone
P85D11B4 (See other available formats)
Regulatory Status
RUO
Other Names
Eukaryotic translation initiation factor 4E, eIF-4E, eIF-4F 25 kD subunit, mRNA cap-binding protein
Isotype
Mouse IgG2a, κ
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Product Citations
publications
P85D11B4_A594_eIF4E_IF_092517
HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.5 µg/mL of Alexa Fluor® 594 anti-eIF4E antibody (clone P85D11B4) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • P85D11B4_A594_eIF4E_IF_092517
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.5 µg/mL of Alexa Fluor® 594 anti-eIF4E antibody (clone P85D11B4) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
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693003 25 µg £94
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Description

The initiation step of protein translation requires a number of eukaryotic translation initiation factors (eIFs). Initially, the 40S ribosomal subunit associates with Met-tRNAi and a group of initiation factors, including eIF1, eIF1A, eIF2, eIF3, and eIF5 to form the 43S pre-initiation complex. This complex is then recruited to the 5'-end of capped mRNA by another complex of factors (known as eIF4F) consisting of eIF4E, eIF4G, and eIF4A. eIF4E is an indispensable element for cap-dependent translation initiation and plays a major role in recognition of the mRNA cap structure. It binds to eIF4G, which serves as a scaffolding protein to gather eIF4A (ATP-dependent RNA helicase), eIF4B, eIF3, and poly-A-binding protein to form the 48S complex. This complex then scans the mRNA for an initiation codon and, once this is located, the 60S ribosomal subunit joins to form the elongation-competent fully functional 80S. eIF4E is the least abundant of the cellular translation initiation components and it represents the rate-limiting element for the initiation process. Under resting conditions, eIF4E is kept in an inactive form bound to 4E-binding proteins (4E-BPs); once the latter have been phosphorylated through the PI3K-AKT-mTOR signaling pathway, eIF4E is released and binds eIF4G to launch translation.

  eIF4E is upregulated in various human tumors, and it contributes to transformation, tumorigenesis, and cancer progression. eIF4E overexpression facilitates translation of weak and highly structured mRNAs that typically encode proteins involved in cancer biology, such as proto-oncoproteins (e.g. cyclin D1, ornithine decarboxylase), angiogenesis factors (e.g. FGF-2, VEGF), and factors related to tumor invasiveness, such as MMP-9. Therefore, eIF4E serves as an attractive cancer drug target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant full length of human eIF4E (AA: 1-217) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Clone P85D11B4 does not cross-react with mouse (in-house tested).

RRID
AB_2650791 (BioLegend Cat. No. 693003)

Antigen Details

Structure
217 amino acids long, and the predicted molecular weight is 25 kD.
Distribution

Cytoplasm

Function
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Interaction
eIF4E is a multi-subunit complex. It is composed of EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners, eg.EIF4ENIF1, EIF4A1, EIF4A2, NGDN, PIWIL2, CYFIP, FMR1, CLOCK, Lassa virus Z protein, MKNK2, LIMD1, WTIP, AJUBA, APOB
Antigen References

1. Kapp LD, Lorsch JR. 2004. Annu. Rev. Biochem. 73: 657.
2. von der Haar T, et al. 2004. Nat. Struct. Mol. Biol. 11: 503.
3. Gingras AC, et al. 1999. Annu. Rev. Biochem. 68: 913.
4. De Benedetti A, Graff JR. 2004. Oncogene 23: 3189.
5. Zimmer SG, et al. 2000. Anticancer Res. 20:1343.
6. Jia Y, et al. 2012. Med. Res. Rev. 32: 786.
7. Graff JR, et al. 2007. J. Clin. Invest. 117: 2638.

Gene ID
1977 View all products for this Gene ID
UniProt
View information about eIF4E on UniProt.org
Go To Top Version: 2    Revision Date: 10/29/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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