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Alexa Fluor® 594 anti-human CD147 Antibody

Pricing & Availability
Clone
A20046A (See other available formats)
Regulatory Status
RUO
Other Names
Neurothelin, Basigin, EMMPRIN
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A20046A_A594_CD147_Antibody_030122
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD147 (clone A20046A) Alexa Fluor® 594 at 4°C overnight. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • A20046A_A594_CD147_Antibody_030122
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD147 (clone A20046A) Alexa Fluor® 594 at 4°C overnight. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
See Alexa Fluor® 594 spectral data
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364603 25 µg £109
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364604 100 µg £265
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Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2910408 (BioLegend Cat. No. 364603)
AB_2910408 (BioLegend Cat. No. 364604)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution

Leukocytes, erythrocytes, platelets, endothelial cells

Function
Adhesion
Antigen References
  1. Biswas C, et al. 1995. Cancer Res. 55:434-9.
  2. Fadool J, et al. 1993. Dev Dyn. 196:252-62.
  3. Felzmann T, et al. 1991. J Clin Immunol. 11:205-12.
Gene ID
682 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org
Go To Top Version: 1    Revision Date: 03/01/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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