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Alexa Fluor® 594 anti-IRF4 Antibody

Pricing & Availability
Clone
IRF4.3E4 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 4
Isotype
Rat IgG1, κ
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Product Citations
publications
IRF4dot3E4_A594_IRF4_Antibody_041718
A20 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 594 rat IgG2a (Cat. No. 400555) or with Alexa Fluor® 594 anti-IRF-4 (red) at (B) 1: 100 (5 µg/ml), (C) 1: 200 (2.5 µg/ml) and (D) 1: 400 (1.25 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • IRF4dot3E4_A594_IRF4_Antibody_041718
    A20 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 594 rat IgG2a (Cat. No. 400555) or with Alexa Fluor® 594 anti-IRF-4 (red) at (B) 1: 100 (5 µg/ml), (C) 1: 200 (2.5 µg/ml) and (D) 1: 400 (1.25 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Compare all formats See Alexa Fluor® 594 spectral data
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646409 25 µg £109
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646410 100 µg £254
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Description

The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
GST fusion protein containing C-terminal of murine IRF4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml (dilution of 1:100-1:400) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Zheng Y, et al. 2009. Nature 458:351
  2. Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed
  3. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  4. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2728475 (BioLegend Cat. No. 646409)
AB_2728475 (BioLegend Cat. No. 646410)

Antigen Details

Structure
Molecular weight is approximately 51 kD.
Distribution

IRF4 is reported to be expressed exclusively in the immune system.

Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lu R. 2008. Trends Immunol 29:487.

Gene ID
3662 View all products for this Gene ID
UniProt
View information about IRF4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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