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Alexa Fluor® 594 anti-MART-1 Antibody

Pricing & Availability
Clone
M2-7C10 (See other available formats)
Regulatory Status
RUO
Other Names
MLANA, Melanoma antigen recognized by T-cells 1, antigen LB39-AA, antigen SK29-AA, protein Melan-A
Isotype
Mouse IgG2b, κ
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Product Citations
publications
M2-7C10_A594_MART-1_Antibody_1_081518
IHC staining of Alexa Fluor® 594 anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. The tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • M2-7C10_A594_MART-1_Antibody_1_081518
    IHC staining of Alexa Fluor® 594 anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. The tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • M2-7C10_A594_MART-1_Antibody_2_081518
    IHC staining of Alexa Fluor® 594 anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. The tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 594 spectral data
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917905 25 µg £94
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917906 100 µg £230
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Description

Melan-A, also known as melanoma antigen recognized by T cells 1 (MART-1), antigen LB39-AA, and antigen SK29-AA, is an 18 kD transmembrane protein that is expressed in a large proportion of melanomas and melanoma cell lines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against a MART-1 fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P).

This antibody recognizes MART-1 (Melan-A) [MART1, MART 1] an 18kD transmembrane protein that is expressed in cells of melanocytic lineage. MART-1 is expressed by a large proportion of melanomas and by melanoma cell lines.

In Immunohistochemistry citrate retrieval has also been verified for use with this clone.

Application References

(PubMed link indicates BioLegend citation)
  1. Shidham VB, et al. 2001. Am. J. Surg. Pathol. 25:1039.
  2. Fetsch PA, et al. 1999. Cancer Cytopath. 87:37.
  3. Yu LL, et al. 1999. Cancer. 86:617.
  4. Beaty MW, et al. 1997. Cancer (Cancer Cytopathology). 81:57.
  5. Marincola FM, et al. 1996. J. Immunother. Emphasis Tumor Immunol. 19:192.
  6. Rivoltini L, et al. 1995. J. Immunol. 154:2257.
  7. Kawakami Y, et al. 1994. J. Exp. Med. 180:347.
  8. Kawakami Y, et al. 1994. Proc. Natl. Acad, Sci. USA. 91:3515.
RRID
AB_2750107 (BioLegend Cat. No. 917905)
AB_2750107 (BioLegend Cat. No. 917906)

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
MHC Antigens
Gene ID
2315 View all products for this Gene ID
UniProt
View information about MART-1 on UniProt.org
Go To Top Version: 1    Revision Date: 06/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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