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Alexa Fluor® 594 anti-mouse CD74 (CLIP) Antibody

Pricing & Availability
Clone
In1/CD74 (See other available formats)
Regulatory Status
RUO
Other Names
CLIP, DHLAG, HLADG
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
In1-CD74_A594_CD74_Antibody_101019
BALB/c mouse was subcutaneous injected with a million of 4T1 (mouse breast cancer) cells on a fat pad. After a week, the spleen was harvested, embedded in OCT and frozen down. The frozen spleen was sectioned, fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 5 µg/mL of CD3 (clone 17A2) Alexa Fluor® 647 (green) and CD74 (clone In1/CD74) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
  • In1-CD74_A594_CD74_Antibody_101019
    BALB/c mouse was subcutaneous injected with a million of 4T1 (mouse breast cancer) cells on a fat pad. After a week, the spleen was harvested, embedded in OCT and frozen down. The frozen spleen was sectioned, fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 5 µg/mL of CD3 (clone 17A2) Alexa Fluor® 647 (green) and CD74 (clone In1/CD74) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 594 spectral data
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151007 25 µg £109
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151008 100 µg £262
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Description

CD74, also known as CLIP, is a 33.5kD homotrimer and functions as a MHC class II chaperone for exogenous peptides and other MHC class II molecules. CD74 binds MIF and regulates innate and adaptive immunity through CD44 signaling. It is found primarily on B-cells, monocytes, macrophages and dendritic cells, but can also be found on activated T-cells, endothelial and epithelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This clone is also known as In1 and In-1. Additional reported applications (for the relevant formats) include: immunohistochemistry1, immunoprecipitation3, and western blotting4.

Application References

(PubMed link indicates BioLegend citation)
  1. Momburg F, et al. 1986. J. Immunol. 136:940. (IHC)
  2. Takahashi K, et al. 2009. Respir. Res. 10:33. (FC)
  3. Meza-Romero R, et al. 2014. J. Immunol. 192:4164. (IP)
  4. Heinrichs D, et al. 2011. Proc. Natl. Acad. Sci. USA 108:17444. (WB)
RRID
AB_2832531 (BioLegend Cat. No. 151007)
AB_2832531 (BioLegend Cat. No. 151008)

Antigen Details

Structure
33.5 kD and homotrimer.
Distribution

B-cells, monocytes/macrophages, dendritic cells, activated T-cells, and activated endothelial/epithelial cells.

Function
MHC class II chaperone, MHC class II molecule sorting, and peptide loading.
Interaction
MHC class II molecules and peptides.
Ligand/Receptor
Macrophage migration inhibitory factor (MIF).
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, MHC Antigens
Antigen References

1. Herrero L, et al. 2013. Arthritis Rheum. 65:2724.
2. Geneve L, et al. 2012. Int. Immunol. 24:645.
3. Sille FC, et al. 2011. Immunol Lett. 139:33.
4. Shih NY, et al. 1995. Mol. Immunol. 32:643.
5. Shi X, et al. 2006. Immunity 25:595.
6. Starlets D, et al. 2006. Blood 107:4807.

Gene ID
16149 View all products for this Gene ID
UniProt
View information about CD74 on UniProt.org
Go To Top Version: 1    Revision Date: 10/10/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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