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Alexa Fluor® 594 anti-Nanog Antibody

Pricing & Availability
Clone
16H3A48 (See other available formats)
Regulatory Status
RUO
Other Names
Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_16H3A48_A594_Nanog_IF_022118
NCCIT cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Nanog (clone 16H3A48) Alexa Fluor® 594 (red) in blocking buffer for three hours, followed by Phalloidin Alexa Fluor® 488 (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • A_16H3A48_A594_Nanog_IF_022118
    NCCIT cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Nanog (clone 16H3A48) Alexa Fluor® 594 (red) in blocking buffer for three hours, followed by Phalloidin Alexa Fluor® 488 (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • B_16H3A48_A594_Nanog_IHCP_022118
    Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human Nanog (clone 16H3A48) antibody (red) at 4°C overnight. The nuclei were conter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 594 spectral data
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674204 100 µg £262
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Description

Nanog is a homeodomain-containing transcription factor that is essential for early embryonic development. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer cell lines, maintaining the undifferentiated status of the cells and is downregulated upon differentiation. Si-RNA mediated knockdown of Nanog in ES cells induces lineage differentiation. Overexpression of Nanog allows for the leukemia inhibitory factor (LIF)-independent self-renewal in undifferentiated ES cells. Nanog functionally works with OCT4 and SOX2 to support pluripotency and self-renewal in ES cells and the expression of Nanog is regulated by OCT4/SOX2 heterodimers. Expression of Nanog has been detected in several types of tumor cells. The expression level of Nanog in cancer cells has been shown to be correlated with the malignancy of cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length NANOG recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2565616 (BioLegend Cat. No. 674204)

Antigen Details

Structure
305 amino acids with a predicted molecular weight of approximately 35 kD and contains a homeobox domain that is responsible for DNA binding.
Interaction
Interacts with ZFP281, SMAD1, and SALL4.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Yin D, et al. 2012. Int. J. Mol. Med. 29:587.
2. Villasante A, et al. 2011. Cell Cycle 10:1488.
3. Theunissen TW, et al. 2011. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366:2222.
4. Pan G, et al. 2007. Cell Res. 17:42.
5. Kashyap V, et al. 2009. Stem Cells Dev. 18:1093.
6. Silva J, et al. 2009. Cell 138:722.

Gene ID
79923 View all products for this Gene ID
UniProt
View information about Nanog on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/20/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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