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Alexa Fluor® 594 anti-SPI1 (PU.1) Antibody

Pricing & Availability
Clone
7C6B05 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen focus forming virus (SFFV) proviral integration oncogene, Transcription factor PU.1
Isotype
Mouse IgG1, κ
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Product Citations
publications
7C6B05_AF594_SPI1_Antibody_1_IF_072414
Human peripheral blood monocyte-derived macrophages were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml of anti-human SPI1 (clone 7C6B05) Alexa Fluor® 594 (red) overnight at 4°C, followed by 10 µg/ml of CD11b Alexa Fluor® 488 (green) staining for 15 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 60X objective.
  • 7C6B05_AF594_SPI1_Antibody_1_IF_072414
    Human peripheral blood monocyte-derived macrophages were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml of anti-human SPI1 (clone 7C6B05) Alexa Fluor® 594 (red) overnight at 4°C, followed by 10 µg/ml of CD11b Alexa Fluor® 488 (green) staining for 15 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 60X objective.
  • 7C6B05_AF594_SPI1_Antibody_2_IHCP_092716
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for 5 minutes, permeabilized with 0.5% Trito-X 100 for ten minutes and blocked with 5% FBS and 0.2% Gelatin for 30 minutes. Then, the tissue was stained with 5 µg per ml of anti-human SPI1 (clone 7C6B05)
Compare all formats See Alexa Fluor® 594 spectral data See high resolution IF/IHC-P data...
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658006 100 µg £262
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Description

SPI1 is a transcription factor belonging to the E26-transformation-specific (Ets) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as occurring malignancy. Overexpression of SPI1 blocks erythroid differentiation and inhibits cell death.  Mice carrying a mutant SPI1 allele show decreased SPI1 expression and develop acute myeloid leukaemia (AML), suggesting the role of SPI1 in oncogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SPI1 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunohistochemisty, a concentration range of 5 - 10 µg per ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

RRID
AB_2563320 (BioLegend Cat. No. 658006)

Antigen Details

Structure
270 amino acids, predicted molecular weight of 31 kD; contains a C-termianl ETS domain responsible for DNA binding.
Distribution

Nucleus.

Function
SPI1 is a transcription factor required for the development of lymphoid and myeloid cells.
Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmelé P, et al. 2010. Cancer Res. 70:6757.

Gene ID
6688 View all products for this Gene ID
UniProt
View information about SPI1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/27/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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