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Alexa Fluor® 594 anti-Synapsin I/II/III Antibody

Pricing & Availability
Clone
A17080A (See other available formats)
Regulatory Status
RUO
Other Names
SYN, Synapsin, Synapsin I, Synapsin II, Synapsin III
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17080A_A594_Synapsin_I-II-III_Antibody_032219.png
IHC staining of Alexa Fluor® 594 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • A17080A_A594_Synapsin_I-II-III_Antibody_032219.png
    IHC staining of Alexa Fluor® 594 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • A17080A_A594_Synapsin_I-II-III_Antibody_2_032219.png
    IHC staining of Alexa Fluor® 594 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
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853707 25 µg £117
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853708 100 µg £241
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Description

Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein corresponding to amino acids 362-450 of human Synapsin I
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 2 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2801205 (BioLegend Cat. No. 853707)
AB_2801205 (BioLegend Cat. No. 853708)

Antigen Details

Structure
Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution

Tissue distribution: Mainly in the central nervous system. Also expressed in testis.
Cellular distribution: Cytosol, nucleus, and plasma membrane.

Function
Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
Interaction
CAPON (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein), PKA
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Greengard P, et al. 1993. Science. 259(5096):780
  2. Milovanovic D, et al. 2017. Neuron. 93(5):995
  3. Evergren E, et al. 2007. J. Neurosci. Res. 85(12):2648
Gene ID
6853 View all products for this Gene ID 6854 View all products for this Gene ID 8224 View all products for this Gene ID
UniProt
View information about Synapsin I/II/III on UniProt.org
Go To Top Version: 1    Revision Date: 03/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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