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Alexa Fluor® 647 anti-AMPKα Phospho (Thr172)

Pricing & Availability
Clone
A20017A (See other available formats)
Regulatory Status
RUO
Other Names
PRKAA1, PRKAA2, AMPKa1, AMPKa2, AMPK, ACACA Kinase, Tau-Protein Kinase PRKAA1, Tau-Protein Kinase PRKAA2, Protein kinase AMP-Activated, Alpha 1 Catalytic Subunit, HMGCR Kinase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A20017A_A-647_AMPKa_IHC-P_070924
IHC staining with Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) on formalin-fixed, paraffin-embedded human colon was performed following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703). Tissue sections were incubated with either Alexa Fluor® 647 Goat anti-mouse only (Cat. No. 405322) (panel A) or with Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) (clone A20017A) (panel B). Nuclei were counterstained with DAPI (Cat. No. 422801). Scale bar: 50 µm
  • A. A20017A_A-647_AMPKa_IHC-P_070924
    IHC staining with Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) on formalin-fixed, paraffin-embedded human colon was performed following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703). Tissue sections were incubated with either Alexa Fluor® 647 Goat anti-mouse only (Cat. No. 405322) (panel A) or with Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) (clone A20017A) (panel B). Nuclei were counterstained with DAPI (Cat. No. 422801). Scale bar: 50 µm
  • B. A20017A_A-647_AMPKa_ICFC_070924
    Serum starved HEK293 cells were either untreated (low expression control, open histogram) or treated with Oligomycin (positive control, filled histogram). The cells were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) (clone A20017A) or Alexa Fluor® 647 mouse IgG1, κ Isotype Control (open histogram, dashed line) (Cat No. 400155).
Compare all formats See Alexa Fluor® 647 spectral data
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600655 25 µg £124
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600656 100 µg £319
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Description

AMP-activated protein kinase alpha (AMPKα) is one subunit of the αβγ heterotrimeric protein complex AMPK. It is a key regulator of metabolism and energy homeostasis in eukaryotes through induction of catabolic pathways and deactivation of anabolic. As an inhibitor of mammalian target of rapamycin complex 1 (mTORC1) pathway, AMPKα is involved in the regulation of cellular growth, cell cycle progression, autophagy, and may play dual roles as both tumor suppressor and promotor. AMPKα is activated via phosphorylation of Threonine residue 172 (Thr172) within the activation loop of the α subunit and is promoted by the allosteric binding of AMP and/or ADP to AMPK’s γ subunit. This phosphorylation is mediated by the serine/threonine kinase liver kinase B1 (LKB1) in conjunction with accessory subunits STRAD and MO25 and also by calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2 aka CAMKKβ). Activation is induced by increases in cellular AMP:ATP and ADP:ATP ratios  following a decline in ATP levels as well as by cellular stress, DNA damage, glucose starvation, and fluxes in calcium and nutrient levels. Localization of AMPKα Thr172 at the spindle poles suggests a novel role for AMPK in mitotic spindle orientation. AMPKα is a key therapeutic target in the treatment of metabolic disorders and an emerging potential treatment target for cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human AMPK alpha phosphorylated at Thr172
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
RRID
AB_3662306 (BioLegend Cat. No. 600655)
AB_3662306 (BioLegend Cat. No. 600656)

Antigen Details

Structure
AMPKα is a 552 amino acid protein with a predicted molecular weight of 63 kD.
Distribution

Heart, Skeletal muscle, Kidney / Nucleus and cytoplasm

Function
Serine/threonine-protein kinase, transcription regulation, Wnt signaling, metabolism
Ligand/Receptor
ATP-binding, magnesium, Metal-binding, Nucelotide-binding
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Mihaylova MM, et al. 2011. Nat Cell Biol 13:1016-23.
  2. Stein SC, et al. 2000. Biochem J. 345:437-43.
  3. Thaiparambil JT, et al. 2012. Mol Cell Biol. 16:3203-17.
  4. Vara-Ciruelos D, et al. 2019. Open Biol. 9:190099.
  5. Willows R, et al. 2017. Biochem J. 474:3059-3073.

 

 

Gene ID
5563 View all products for this Gene ID 5562 View all products for this Gene ID
UniProt
View information about AMPKalpha Phospho Thr172 on UniProt.org
Go To Top Version: 1    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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