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Alexa Fluor® 647 anti-FosB Antibody

Pricing & Availability
Clone
W19011B (See other available formats)
Regulatory Status
RUO
Other Names
G0/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W19011B_-Alexa-Fluor-647_FOSB_ICFC_033123
HeLa cells untreated (negative control, open histogram) or treated with 100 ng/mL PMA for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-FosB (clone W19011B) or Alexa Fluor® 647 rat IgG2a, κ isotype control (open histogram, dashed line) (representative histogram for untreated and treated cells) (Cat. No. 400526).
  • a. W19011B_-Alexa-Fluor-647_FOSB_ICFC_033123
    HeLa cells untreated (negative control, open histogram) or treated with 100 ng/mL PMA for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-FosB (clone W19011B) or Alexa Fluor® 647 rat IgG2a, κ isotype control (open histogram, dashed line) (representative histogram for untreated and treated cells) (Cat. No. 400526).
  • b. W19011B_-Alexa-Fluor-647_FOSB_ICC_033123
    HeLa cells untreated (panels A and B) or treated with 100 ng/mL of PMA (panels C and D) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with 5.0 µg/mL of Alexa Fluor® 647 anti-FosB (clone W19011B) (red) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Panels A and C represent Alexa Fluor® 647 channel; panels B and D represent merged Alexa Fluor® 647 and DAPI channels. Images were captured with a 60X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
600853 25 tests £126
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600856 100 µg £254
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600854 100 tests £262
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Description

FosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant FosB protein
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC (test sizes) - Quality tested
ICC (µg size) - Quality tested
Recommended Usage

Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Methanol only and PFA + Methanol fix/perm methods were tested and produced inferior signal.

ICFC: Cells were fixed and permeabilizes using True Phos Buffer system

ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100 

 

RRID
AB_2941600 (BioLegend Cat. No. 600853)
AB_2941600 (BioLegend Cat. No. 600856)
AB_2941600 (BioLegend Cat. No. 600854)

Antigen Details

Structure
FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.
Distribution

FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.

Function
FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.
Interaction
Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G/C TCA) SWI/SNF chromatin remodeling complex, DPP4
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Tumor Suppressors
Antigen References
  1. Nestler E. 2015. Eur J Pharmacol. 753:66-72.
  2. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.
Gene ID
2354 View all products for this Gene ID
UniProt
View information about FosB on UniProt.org

Other Formats

View All FosB Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-FosB W19011B IHC-P,ICC,ICFC
Alexa Fluor® 647 anti-FosB W19011B ICFC,ICC
PE anti-FosB W19011B ICFC
Go To Top Version: 1    Revision Date: 03/31/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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