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Alexa Fluor® 647 anti-γ-Catenin Antibody

Pricing & Availability
Clone
15F11 (See other available formats)
Regulatory Status
RUO
Other Names
Junction Plakoglobin, DP3, Catenin (Cadherin-associated Protein), Gamma 80kDa, Desmoplakin III, CTNNG, PDGB, PKGB, Catenin Gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
15F11_Alexa-Fluor-647_gamma-Catenin_Antibody_102522
MCF7 cells (positive control) (filled histogram) and MOLT-4 cells (negative control) (open histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-γ Catenin (clone 15F11), or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400136).
  • 15F11_Alexa-Fluor-647_gamma-Catenin_Antibody_102522
    MCF7 cells (positive control) (filled histogram) and MOLT-4 cells (negative control) (open histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-γ Catenin (clone 15F11), or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400136).
Compare all formats See Alexa Fluor® 647 spectral data
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946403 25 tests £126
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946404 100 tests £262
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Description

γ-catenin, also referred to as JUP and plakoglobin, is a member of the Armadillo family of proteins that are required for Wnt signaling. γ-catenin was originally described as a structural component of desmosomes and adherens junctions, where it links E-cadherins to the actin cytoskeleton. The presence of this complex is critical for cell migration and adhesion. While γ-catenin shows high overall similarity to the homolologous β-catenin, notably in the armadillo domain rich central region, γ-catenin has distinct N- and C-terminal regions, which allows interaction with protein partners functionally distinct with those of other catenins. Additionally, γ-catenin shows tumor suppressor activity by downregulation of oncogenic Wnt signaling. This negative regulation occurs through multiple mechanisms: a. displacement of β-catenin from junctional complexes thereby promoting its ubiquitin-dependent degradation, b. disrupting formation of the TCF- β-catenin transcriptional activator complex by directly binding β-catenin, c. stimulating the nuclear export of the SOX4 transcription factor. γ-catenin also plays a role in maintaining embryonic stem cells in a pluripotent state.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant chicken γ-catenin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This clone was tested for ICC on MCF7 (positive control) and MOLT-4 (negative control) cells using multiple fixation and permeabilization conditions. A brief summary of these results is listed below:

  • methanol only: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Moderate nuclear background staining observed in MCF7 cells.
     
  • 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
     
  • 4% PFA plus methanol: 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
RRID
AB_2927949 (BioLegend Cat. No. 946403)
AB_2927949 (BioLegend Cat. No. 946404)

Antigen Details

Structure
γ-catenin is a 745 amino acid protein with a predicted molecular weight of 82 kD.
Distribution

Ubiquitously expressed/Cytoskeleton and plasma membrane associated

Function
Cell to cell adhesion, cell signaling
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Signal Transduction
Antigen References
  1. Zhurinksy J, et al. 2000. J Cell Sci. 18:3127.
  2. Mahendram S, et al. 2013. PLoS ONE. 8:e65320.
  3. Aktary Z, et al. 2017. Oncotarget. 8:32270.
Gene ID
3728 View all products for this Gene ID
UniProt
View information about gamma-Catenin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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