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Alexa Fluor® 647 anti-human Basophils (2D7) Antibody

Pricing & Availability
Clone
Basophil/2D7 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
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Product Citations
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Basophilslash2D7_A647_030510
Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor® 647
  • Basophilslash2D7_A647_030510
    Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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346204 100 tests £238
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Description

The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Lysates of purified basophils
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.

Application References

(PubMed link indicates BioLegend citation)
  1. Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
  2. Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
  3. Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
  4. Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
  5. Sperr WR, et al. 2001. Blood 98:2200. (IHC)
  6. Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
  7. Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
RRID
AB_1967142 (BioLegend Cat. No. 346204)

Antigen Details

Distribution

Basophils.

Cell Type
Basophils
Biology Area
Immunology, Innate Immunity
Gene ID
NA
UniProt
View information about Basophil on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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