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Alexa Fluor® 647 anti-human CD133 Antibody

Pricing & Availability
Clone
S16015F (See other available formats)
Regulatory Status
RUO
Other Names
Prominin-1, PROM1, AC133
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S16015F_A647_CD133_Antibody_071724
Human peripheral blood mononuclear cells were stained with anti-human CD34 (clone 561) PE and anti-human CD133 (clone S16015F) Alexa Fluor® 647 (left) or, mouse IgG2a, κ Alexa Fluor® 647 isotype control (right). Data shown were gated on the CD45+ CD14- lymphocyte population.
  • S16015F_A647_CD133_Antibody_071724
    Human peripheral blood mononuclear cells were stained with anti-human CD34 (clone 561) PE and anti-human CD133 (clone S16015F) Alexa Fluor® 647 (left) or, mouse IgG2a, κ Alexa Fluor® 647 isotype control (right). Data shown were gated on the CD45+ CD14- lymphocyte population.
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393913 25 tests £117
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393914 100 tests £257
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Description

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD133 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

In-house testing suggests that clone S16015F does not block clones 293C3, AC133, and clone 7 that are also raised against human CD133.

RRID
AB_3662291 (BioLegend Cat. No. 393913)
AB_3662291 (BioLegend Cat. No. 393914)

Antigen Details

Structure
120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains
Distribution

Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells

Function
May play a role in cell differentiation, proliferation, and apoptosis
Interaction
NAT8; NAT8B; CDHR1
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Yin AH, et al. 1997. Blood. 90:5002.
  2. Miraglia S, et al. 1997. Blood. 90:5013.
  3. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
Gene ID
8842 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org
Go To Top Version: 1    Revision Date: 07/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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