Alexa Fluor 647 anti-human CD174 Antibody anti-CD174

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Clone: NCRC37 (See other available formats)
Regulatory Status: RUO
Other Names: SC104
Isotype: Mouse IgG1, κ
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NCRC37_A647_CD174_Antibody_1_071118 — Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes permeabilized with 0.5% TritonX-100 in PBS for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 goat anti-Vimentin (Clone O91D3) antibody (green) and Alexa Fluor® 647 anti-human CD174 (Clone NCRC37) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.

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392605

25 µg

£109

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Description

CD174 is also known as Lewis Y, Fucosyltransferase 3, Galactoside 3(4)-L-fucosyltransferase which is encoded by FUT3 gene. FUT3 protein has 361a.a., around 39.7 kD and is highly expressed on duodenum and small intestine. The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Tissue cultured colorectal tumor cell line (C170, C146, COLO205, and JW)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2749948 (BioLegend Cat. No. 392605)

Antigen Details

Distribution: Highly expressed on glandular cells of duodenum and small intestine.
Function: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme.
Biology Area: Cancer Biomarkers, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Durrant, LG. et al. 2006. Cancer Res. 66:5901-9
Gene ID: 2525 View all products for this Gene ID
UniProt: View information about CD174 on UniProt.org

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Other Formats

View All CD174 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD174	NCRC37	FC,IHC-P
Alexa Fluor® 594 anti-human CD174	NCRC37	IHC-P
Alexa Fluor® 647 anti-human CD174	NCRC37	IHC-P,FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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