- Clone
- S16021I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SLE1, TIL3, SLEB1, MELIOS, CD285
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S16021I_A647_CD285_Antibody_100819.png S16021I_A647_CD285_Antibody_100819.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S16021I_A647_CD285_Antibody_100819.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stained with CD14 FITC and CD285 (TLR5) (clone S16021I) Alexa Fluor® 647 (left) or Mouse IgG2a, κ Alexa Fluor® 647 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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394505 | 25 tests | £113 | ||||
394506 | 100 tests | £257 |
CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TLR5 transfectants
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- RRID
-
AB_2820040 (BioLegend Cat. No. 394505)
AB_2820041 (BioLegend Cat. No. 394506)
Antigen Details
- Structure
- Type 1 transmembrane protein, 98 kD
- Distribution
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Neutrophils, classical monocytes, dendritic cells, mucosal epithelium
- Function
- Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
- Ligand/Receptor
- flagellin
- Cell Type
- Dendritic cells, Epithelial cells, Monocytes, Neutrophils
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
-
- Hayashi F, et al. 2001. Nature. 410:1099
- Gewirtz AT, et al. 2001. J Immunol. 167:1882
- Smith KD, et al. 2002. Curr Top Microbiol Immunol. 270:93
- Bambou JC, et al. 2004. J Biol Chem. 279:42984
- Sebastiani G, et al. 2000. Genomics. 64:230
- Muzio M, et al. 2000. Biochem Soc Trans. 28:563
- Gene ID
- 7100 View all products for this Gene ID
- UniProt
- View information about CD285 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD285 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD285 (TLR5) | S16021I | FC |
PE anti-human CD285 (TLR5) | S16021I | FC |
Alexa Fluor® 647 anti-human CD285 (TLR5) | S16021I | FC |
APC anti-human CD285 (TLR5) | S16021I | FC |
Alexa Fluor® 488 anti-human CD285 (TLR5) | S16021I | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD285 (TLR5)
Human peripheral blood monocytes were stained with CD14 APC ... -
PE anti-human CD285 (TLR5)
Human peripheral blood monocytes were stained with CD14 APC ... -
Alexa Fluor® 647 anti-human CD285 (TLR5)
Human peripheral blood monocytes were stained with CD14 FITC... -
APC anti-human CD285 (TLR5)
Human peripheral blood monocytes were stained with CD14 Alex... -
Alexa Fluor® 488 anti-human CD285 (TLR5)
Human peripheral blood monocytes were stained with CD14 APC ...
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