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Alexa Fluor® 647 anti-human IgD Antibody

Pricing & Availability
Clone
W18340A (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18340A_A647_IgD_Antibody_120523
IHC staining of anti-human IgD (clone W18340A) Alexa Fluor® 647 on formalin-fixed paraffin-embedded human tonsil tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human IgD (clone W18340A) Alexa Fluor® 647 (red) and anti-human CD45RA (clone HI100) Alexa Fluor® 594 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
  • W18340A_A647_IgD_Antibody_120523
    IHC staining of anti-human IgD (clone W18340A) Alexa Fluor® 647 on formalin-fixed paraffin-embedded human tonsil tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human IgD (clone W18340A) Alexa Fluor® 647 (red) and anti-human CD45RA (clone HI100) Alexa Fluor® 594 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
See Alexa Fluor® 647 spectral data
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324503 25 µg £76
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324504 100 µg £189
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Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length human IgD recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
RRID
AB_3097334 (BioLegend Cat. No. 324503)
AB_3097334 (BioLegend Cat. No. 324504)

Antigen Details

Structure
Exists in a transmembranal and a soluble form
Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Interaction
The CD79a/CD79b heterodimer
Molecular Family
Immune Checkpoint Receptors, Innate Immune Signaling
Antigen References
  1. Geisberger R, et al. 2006. Immunology 118:429-37.
  2. Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
  3. Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/05/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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