Alexa Fluor 647 anti-human IL-9 Antibody anti-IL-9

Pricing & Availability

Clone: MH9A4 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype: Mouse IgG2b, κ
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PMA and ionomycin-stimulated (6 hours, in the presence of monensin) Th2-polarized human lymphocytes were surface stained with CD3 FITC. Cells were fixed, permeabilized and intracellularly stained with IL-9 (clone MH9A4) Alexa Fluor® 647 (left), or mouse IgG2b, κ Alexa Fluor® 647 isotype control (right).

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507607

25 tests

£101

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507608

100 tests

£221

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Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macropahges and induce TGF-β production. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Baculovirus-expressed, recombinant human IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: ELISA Capture²: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 antibody as the detecting antibody.
For human IL-9 neutralization assay, the LEAF™ purified MH9D1 antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-9 bioactivity.
RRID: AB_2233829 (BioLegend Cat. No. 507607)
AB_2233829 (BioLegend Cat. No. 507608)

Antigen Details

Structure: Cytokine; 32-39 kD (Mammalian)
Bioactivity: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources: IL-2 activated T_H2 lymphocytes, Hodgkin's lymphoma
Cell Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441.
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID: 3578 View all products for this Gene ID
UniProt: View information about IL-9 on UniProt.org

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Other Formats

View All IL-9 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human IL-9	MH9A4	ELISA Capture
PE anti-human IL-9	MH9A4	ICFC
Alexa Fluor® 647 anti-human IL-9	MH9A4	ICFC
PerCP/Cyanine5.5 anti-human IL-9	MH9A4	ICFC
PE/Cyanine7 anti-human IL-9	MH9A4	ICFC
APC anti-human IL-9	MH9A4	ICFC
TotalSeq™-B1151 anti-human IL-9	MH9A4	ICPG

Certificate of Analysis

Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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