Alexa Fluor® 647 anti-human LYPD3 Antibody, LYPD3, 11A1

Pricing & Availability

Clone: 11A1 (See other available formats)
Regulatory Status: RUO
Other Names: C4.4A, GPI-Anchored Metastasis-Associated Protein C4.4A Homolog, LY6/PLAUR Domain Containing 3
Isotype: Mouse IgG2b, κ
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Product Citations: publications

A.
11A1_ALEXA-647_LYPD3_IHC-Antibody_010424 — IHC staining of Alexa Fluor® 647 anti-human LYPD3 (clone 11A1). A) Positive on formalin-fixed paraffin-embedded skin tissue and B) Negative on formalin-fixed paraffin-embedded brain tissue. Following antigen retrieval using 1X Citrate buffer 1X pH 6.0 (Cat. No. 420902). The tissue was incubated with 10 µg/mL of Alexa Fluor® 647 anti-human LYPD3 (clone 11A1) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective.

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364505

25 µg

£165

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364506

100 µg

£325

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Description

C4.4A, also known as Ly6/PLAUR domain containing 3 (LYPD-3), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR). C4.4A is also expressed in the placenta, skin and urothelium and can be found in suprabrasal layers of stratified squamous epithelium and is upregulated on migrating keratinocytes during wound healing. Human C4.4A mRNA has been detected in cancer cell lines, including melanoma, breast, bladder, and renal cell carcinoma, as well as in tumor tissue samples from malignant melanoma, colorectal cancer (CRC), breast cancer, lung carcinoma, and urothelial tumors. Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_3097239 (BioLegend Cat. No. 364505)
AB_3097239 (BioLegend Cat. No. 364506)

Antigen Details

Distribution: Placenta, skin, urothelium, keratinocytes
Function: Interacting selectively and non-covalently with laminins, glycoproteins that are major constituents of the basement membrane of cells
Interaction: LGALS3 (By similarity). AGR2 and AGR3
Ligand/Receptor: Laminin-1 and laminin-5
Biology Area: Cell Adhesion
Molecular Family: Adhesion Molecules
Antigen References: Smith BA, et al. 2001. Cancer Res. 61:1678-85.

Würfle J, et al. 2001. Gene. 262:35-41.
Gene ID: 27076 View all products for this Gene ID
UniProt: View information about LYPD3 on UniProt.org

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Other Formats

View All LYPD3 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human LYPD3	11A1	IHC-P,FC
Alexa Fluor® 594 anti-human LYPD3	11A1	IHC-P
Alexa Fluor® 647 anti-human LYPD3	11A1	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/15/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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