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Alexa Fluor® 647 anti-human/mouse/rat PCNA Antibody

Pricing & Availability
Clone
PC10 (See other available formats)
Regulatory Status
RUO
Other Names
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PC10_Alx647_071007
Human peripheral blood lymphocytes fixed with 70% ethanol, then intracellular stained with PC10 Alexa fluor® 647
  • PC10_Alx647_071007
    Human peripheral blood lymphocytes fixed with 70% ethanol, then intracellular stained with PC10 Alexa fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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307912 100 tests £182
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Description

The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant rat PCNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.

Application References

(PubMed link indicates BioLegend citation)
  1. Ogata K, et al. 1985. J. Immunol. 135:2623.
  2. Garcia R, et al. 1989. Am. J. Pathol. 134:733.
  3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
  4. Waseem N, et al. 1990. J. Cell Sci. 96:121.
  5. Yu C, et al. 1991. Histopathology 19:29.
  6. Wilkins B, et al. 1992. J. Pathol. 166:45.
  7. Yang W, et al. 1996. Human Pathol. 27:70.
  8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
  10. Fulvio MD, et al. 2006. Oncogene 25:3932.
  11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
  12. Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
  13. Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
  14. Wallace HA, et al. 2014. Development. 141:1332. PubMed
  15. Mizokami A, et al. 2014. Bone. 69:68. PubMed
Product Citations
  1. Ritzel RM, et al. 2022. Geroscience. 44:1407. PubMed
  2. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
RRID
AB_2267947 (BioLegend Cat. No. 307912)

Antigen Details

Structure
DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution

Nuclear, all proliferating cells

Function
RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Interaction
PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Ligand/Receptor
Ubiquitination, Sumoylation
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Nuclear Markers
Antigen References

1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.

Gene ID
5111 View all products for this Gene ID
UniProt
View information about PCNA on UniProt.org
Go To Top Version: 3    Revision Date: 11/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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