Alexa Fluor 647 anti-human Sialyl Lewis X dimeric Antibody anti-Sialyl Lewis X

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Clone: FH6 (See other available formats)
Regulatory Status: RUO
Other Names: CD15s, Stage-Specific Embryonic Antigen 1, SSEA-1, Sialyl Lewis X, sLex, sLe(x), Sialyl demeric LeX, Sialyl dermic Lewis X, Sialyl Lewis X-i, sLeX-i
Isotype: Mouse IgM, κ
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Product Citations: publications

FH6_AF647_SialylLewisX_Antibody_1_FC_081915 — Human peripheral blood granulocytes were stained with sialyl lewis X (dimeric) (clone FH6) Alexa Fluor® 647 (filled histogram) or mouse IgM, κ Alexa Fluor® 647 isotype control (open histogram).

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368103

25 tests

£109

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Description

The FH6 antibody recognizes Sialyl Lewis X (demeric) on glycolipids or glycoproteins. It also recognizes Sialyl Lewis X with long carbohydrate attachments (Sialyl Lewis X-i). These antigens are expressed on human granulocytes, monocytes, small subsets of lymphocytes, some fetal tissues such as the fetal stomach, fetal colon, and fetal intestine, and a variety of cancer tissues. It is believed that these antigens are involved in cell adhesion.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified 6B fucoganglioside absorbed to Salmonella minnesota.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2566070 (BioLegend Cat. No. 368103)

Antigen Details

Structure: Carbohydrate epitope; VI³NeuAcV³III³Fuc₂nLc₆.
Distribution: Granulocytes, monocytes, subpopulations of lymphocytes, fetal stomach, fetal colon, fetal small intestine, and a variety of cancer tissues.
Function: Cell adhesion.
Ligand/Receptor: Selectins.
Cell Type: Embryonic Stem Cells, Granulocytes, Lymphocytes, Monocytes, Neural Stem Cells, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511.
2. Kannagi R, et al. 1986. Cancer Research 5:2619.
3. Nakasaki H, et al. 1989. Cancer Research 49:3662.
4. Dohi T, et al. 1993. Anticancer Res. 13:1277.
Gene ID: 2526 View all products for this Gene ID
UniProt: View information about Sialyl Lewis X on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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View All Sialyl Lewis X (dimeric) Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 488 anti-human Sialyl Lewis X (dimeric)	FH6	FC
Purified anti-human Sialyl Lewis X (dimeric)	FH6	FC,IHC,RIA
Alexa Fluor® 647 anti-human Sialyl Lewis X (dimeric)	FH6	FC
FITC anti-human Sialyl Lewis X (dimeric)	FH6	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/21/2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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