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Alexa Fluor® 647 anti-human TCL1 Antibody

Pricing & Availability
Clone
1-21 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell leukemia/lymphoma protein 1A, Protein p14 TCL1, Oncogene TCL1, TCL-1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1-21_A647_110308.jpg
Human peripheral blood lymphocytes stained with anti-CD19 (HIB19) PE then intracellularly stained with 1-21 Alexa Fluor® 647
  • 1-21_A647_110308.jpg
    Human peripheral blood lymphocytes stained with anti-CD19 (HIB19) PE then intracellularly stained with 1-21 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
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330508 100 tests £211
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Description

TCL1, also known as T-cell leukemia/lymphoma protein 1A, protein p14 TCL1 and oncogene TCL-1, is a 14kD beta-barrel protein that was originally identified on the basis of its association with T-cell prolymphocytic leukemia (T-PLL). Activated by the T-cell receptor enhancer through inv(14)(q11;q32.1) or t(14;14)(q11;q32.1), TCL1 is normally expressed in immature cortical thymocytes, activated peripheral T-cells,  pro-B cells, and naive mantle zone B cells of peripheral lymphoid tissues, but not in post-germinal center (GC) memory B cells and plasma cells. Akt and TCL1 interact via Akt’s pleckstrin homology domain to form hetero-oligomers at the leaflet of the inner plasma membrane stimulating the protein kinase C-mitogen activated protein kinase-extracellular signal-related kinase (PKC-MAPK-ERK) signal transduction pathway. TCL1 also inhibits activation-induced cell death in T-cells by blocking PKCθ. In addition to T-PLL dysregulation is implicated in such B-cell neoplasias as lymphoblastic lymphoma, chronic lymphocytic leukemia (CLL) and Burkitt lymphoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
outer α-loop region of TCL1 peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunoprecipitation, western blot, immunohistochemical staining of frozen or formalin-fixed, paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Herling M, et al. 2008. Blood 111:328
  2. Herling M, et al. 2007. Am. J. Surg. Pathol. 31:1123
Product Citations
  1. Bunse M, et al. 2021. Nat Commun. 0.666666667. PubMed
RRID
AB_2204405 (BioLegend Cat. No. 330508)

Antigen Details

Structure
Beta barrel protein, 14kD
Distribution
Cytoplasm
Function
Enhances Akt mediated cell survival, stabilizes mitochondrial membrane potential
Interaction
Akt1, Akt2, Akt3, caco2
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Narducci MG et al. 2000. Cancer Research 60:2095.
2. Teitell MA et al. 2005 Nat. Rev. Cancer 5:640
3. Laine J et al.2000. Molecular Cell 6:395
4. Virgilio L et al. 1994. Proc. Natl. Acad. Sci. 91:12530
5. Masayuk N et al. 2007 FASEB J. 21:2273
6. Despouy G et al. 2007 Blood 110:4406
7. Rhine S et al. 2006 Blood 108:1991

Gene ID
8115 View all products for this Gene ID
UniProt
View information about TCL1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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