- Clone
- 3C10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell antigen receptor Vα7.2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3C10_AF647_TCRVa7.2_Antibody_FC_1_092315 3C10_AF647_TCRVa7.2_Antibody_FC_1_092315](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3C10_AF647_TCRVa7.2_Antibody_FC_1_092315.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD3 PE and TCR Vα7.2 (clone 3C10) Alexa Fluor® 647 (top), or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom). -
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Confocal image of human lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: HLA-DR (purple) in Cycle 3 and Vα7.2 (yellow) in Cycle 7. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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351725 | 25 tests | £109 | ||||
351726 | 100 tests | £254 |
The 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa. MAIT cells play a role in detecting and fighting off microbial infections.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant TCR
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
SB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
- Additional Product Notes
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Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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Application References
(PubMed link indicates BioLegend citation) - RRID
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AB_2566334 (BioLegend Cat. No. 351725)
AB_2566335 (BioLegend Cat. No. 351726)
Antigen Details
- Structure
- Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
- Distribution
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Mucosal-associated invariant T (MAIT) cells and conventional T cells
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
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1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
- Gene ID
- 6955 View all products for this Gene ID
- UniProt
- View information about TCR Valpha7.2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
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It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
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Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
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Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
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Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
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In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All TCR Vα7.2 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human TCR Vα7.2 | 3C10 | FC |
FITC anti-human TCR Vα7.2 | 3C10 | FC |
PE anti-human TCR Vα7.2 | 3C10 | FC |
APC anti-human TCR Vα7.2 | 3C10 | FC |
PerCP/Cyanine5.5 anti-human TCR Vα7.2 | 3C10 | FC |
PE/Cyanine7 anti-human TCR Vα7.2 | 3C10 | FC |
APC/Cyanine7 anti-human TCR Vα7.2 | 3C10 | FC |
Brilliant Violet 421™ anti-human TCR Vα7.2 | 3C10 | FC |
Brilliant Violet 510™ anti-human TCR Vα7.2 | 3C10 | FC |
Brilliant Violet 605™ anti-human TCR Vα7.2 | 3C10 | FC |
Brilliant Violet 785™ anti-human TCR Vα7.2 | 3C10 | FC |
Biotin anti-human TCR Vα7.2 | 3C10 | FC |
Alexa Fluor® 647 anti-human TCR Vα7.2 | 3C10 | FC,SB |
Alexa Fluor® 700 anti-human TCR Vα7.2 | 3C10 | FC |
PE/Dazzle™ 594 anti-human TCR Vα7.2 | 3C10 | FC |
Brilliant Violet 711™ anti-human TCR Vα7.2 | 3C10 | FC |
TotalSeq™-A0581 anti-human TCR Vα7.2 | 3C10 | PG |
TotalSeq™-C0581 anti-human TCR Vα7.2 | 3C10 | PG |
TotalSeq™-B0581 anti-human TCR Vα7.2 | 3C10 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TCR Vα7.2
Human peripheral blood lymphocytes stained with CD3 APC and ... -
FITC anti-human TCR Vα7.2
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PE anti-human TCR Vα7.2
Human peripheral blood lymphocytes stained with CD3 APC and ... -
APC anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PerCP/Cyanine5.5 anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 APC... -
PE/Cyanine7 anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC/Cyanine7 anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 510™ anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 605™ anti-human TCR Vα7.2
Human peripheral blood lymphocytes stained with CD3 FITC and... -
Brilliant Violet 785™ anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 APC... -
Biotin anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 APC... -
Alexa Fluor® 647 anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 PE ... Confocal image of human lymph node sample acquired using the... -
Alexa Fluor® 700 anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 and... -
PE/Dazzle™ 594 anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 711™ anti-human TCR Vα7.2
Human peripheral blood lymphocytes were stained with CD3 FIT... -
TotalSeq™-A0581 anti-human TCR Vα7.2
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TotalSeq™-C0581 anti-human TCR Vα7.2
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TotalSeq™-B0581 anti-human TCR Vα7.2
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