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Alexa Fluor® 647 anti-mouse CD106 Antibody

Pricing & Availability
Clone
429 (MVCAM.A) (See other available formats)
Regulatory Status
RUO
Other Names
VCAM-1, INCAM-110
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_429(MVCAM.A)_AF647_CD106_Antibody_1_FC_011108
C57BL/6 bone marrow myeloid cells stained with 429 Alexa Fluor® 647
  • 1_429(MVCAM.A)_AF647_CD106_Antibody_1_FC_011108
    C57BL/6 bone marrow myeloid cells stained with 429 Alexa Fluor® 647
  • 2_429(MVCAM.A)_AF647_CD106_Antibody_2_IHCF_092016
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 647 (green) and 10 µg/mL of CD117 (clone 2B8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 3_429(MVCAM.A)_AF647_CD106_Antibody_3_IHCF_032719
    Dissected C57/B6 mouse testis was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 5 µg/mL of anti-mouse CD147 (clone OX-114) Alexa Fluor® 594 (green) and CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 647 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 4_429(MVCAM.A)_AF647_CD106_Antibody_3_IHCF_032719
    Formalin-fixed, 300 micron-thick mouse testis section was blocked, permeabilized and stained overnight with CD106 (clone 429, MCVAM.A) Alexa Fluor® 647 (red), and CD147 (clone OX-114) Alexa Fluor® 488 (green) both at 5 µg/mL, optically cleared, and analyzed at 225 µm imaging depth on a confocal microscope. Watch the video.
  • 5_429-MVCAM-A_A647_CD106_Antibody_5_091721.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse CD147 Antibody (clone OX-114) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD117 (c-Kit) Antibody (clone 2B8) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD106 Antibody (clone 429 (MVCAM.A)) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
Compare all formats See Alexa Fluor® 647 spectral data See high resolution IHC-F data...
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105711 25 µg £70
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105712 100 µg £157
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Description

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse preadipose cell line PA6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 2.5 - 10 µg/mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
  2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
  4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
  6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
  7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
  9. Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
  10. Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  2. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  3. Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
  4. Adamiak M, et al. 2015. Leukemia. . PubMed
  5. Sung SJ, et al. 2020. Bio Protoc. 10:e3663. PubMed
  6. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  7. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  8. Nakagawa R, et al. 2013. J Immunol. 190:3309. PubMed
  9. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  10. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  11. Bolkhovitina EL, et al. 2022. Int J Mol Sci. 23: . PubMed
  12. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  13. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  14. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  15. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
RRID
AB_493430 (BioLegend Cat. No. 105711)
AB_493430 (BioLegend Cat. No. 105712)

Antigen Details

Structure
Ig superfamily, 47 kD
Distribution

Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Adhesion
Ligand/Receptor
VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD106 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD106 429 (MVCAM.A) FC
FITC anti-mouse CD106 429 (MVCAM.A) FC
LEAF™ Purified anti-mouse CD106 429 (MVCAM.A) FC, IHC-F, IP
Purified anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,IP
Alexa Fluor® 488 anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,SB
Alexa Fluor® 647 anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,3D IHC
PE anti-mouse CD106 429 (MVCAM.A) FC
PerCP/Cyanine5.5 anti-mouse CD106 429 (MVCAM.A) FC
APC anti-mouse CD106 429 (MVCAM.A) FC
PE/Cyanine7 anti-mouse CD106 429 (MVCAM.A) FC
Pacific Blue™ anti-mouse CD106 429 (MVCAM.A) FC
Alexa Fluor® 594 anti-mouse CD106 429 (MVCAM.A) IHC-F
TotalSeq™-A0226 anti-mouse CD106 429 (MVCAM.A) PG
Ultra-LEAF™ Purified anti-mouse CD106 429 (MVCAM.A) FC,IHC-F,IP
TotalSeq™-C0226 anti-mouse CD106 429 (MVCAM.A) PG
TotalSeq™-B0226 anti-mouse CD106 429 (MVCAM.A) PG
Go To Top Version: 7    Revision Date: 04/13/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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