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Alexa Fluor® 647 anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
a-2B8_Alx647_A_020708
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
  • a-2B8_Alx647_A_020708
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
  • b-2B8_Alx647_B_020708
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 647
  • c-2B8_A647_CD117_Antibody_3_110419
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-mouse CD117 (clone 2B8) Alexa Fluor® 647 (red), and anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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105817 25 µg £70
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105818 100 µg £157
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Barkaway A, et al. 2021. Immunity. . PubMed
  2. Olsen SN, et al. 2022. Mol Cell. 82:1140. PubMed
  3. Hodzic D, et al. 2022. PLoS Biol. 20:e3001811. PubMed
  4. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  5. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  6. Harhouri K, et al. 2010. Blood. 115:3843. PubMed
  7. Amouzegar A, et al. 2017. Stem Cells. 10.1002/stem.2611. PubMed
  8. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  9. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  10. Hoshii T, et al. 2018. Cell. 172:1007. PubMed
  11. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_493474 (BioLegend Cat. No. 105817)
AB_493474 (BioLegend Cat. No. 105818)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 4    Revision Date: 11/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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