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Alexa Fluor® 647 anti-mouse CD146 Antibody

Pricing & Availability
Clone
A20078A (See other available formats)
Regulatory Status
RUO
Other Names
S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1. A20078A_A647_CD146_Antibody_1_072823
C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.1 FITC and anti-mouse CD146 (clone A20078A) Alexa Fluor® 647 (left), or rat IgG2b, κ Alexa Fluor® 647 isotype control (right).
  • 1. A20078A_A647_CD146_Antibody_1_072823
    C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.1 FITC and anti-mouse CD146 (clone A20078A) Alexa Fluor® 647 (left), or rat IgG2b, κ Alexa Fluor® 647 isotype control (right).
  • 2. A20078A_A647_CD146_Ab_2_082423
    IHC staining of anti-mouse CD146 (clone A20078A) Alexa Fluor® 647 on frozen mouse kidney section. C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was stained with 5 µg/mL of anti-mouse Podoplanin Alexa Fluor® 488 (green) and 5 µg/mL of anti-mouse CD146 (clone A20078A) Alexa Fluor® 647 (red) (panel A) or rat IgG2b, κ Alexa Fluor® 647 isotype control (red) (panel B) at 4°C overnight. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
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112005 25 µg £93
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112006 100 µg £195
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Description

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFN-γ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein (val 24-val 563 extracellular domain)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_3068233 (BioLegend Cat. No. 112005)
AB_3068233 (BioLegend Cat. No. 112006)

Antigen Details

Structure
CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
Distribution

CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.

Function
CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
Ligand/Receptor
Unidentified ligand
Cell Type
Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
84004 View all products for this Gene ID
UniProt
View information about CD146 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/28/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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