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Alexa Fluor® 647 anti-mouse CD182 (CXCR2) Antibody

Pricing & Availability
Clone
SA045E1 (See other available formats)
Regulatory Status
RUO
Other Names
IL-8RB, GRO/MGSA, Cmkar2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
SA045E1_AF647_CD182_Antibody_FC_1_021815
C57BL/6 mouse bone marrow cells were costained with Ly-6G/Ly-6C FITC and CXCR2 (clone SA045E1) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • SA045E1_AF647_CD182_Antibody_FC_1_021815
    C57BL/6 mouse bone marrow cells were costained with Ly-6G/Ly-6C FITC and CXCR2 (clone SA045E1) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • SA045E1_AF647_CD182_Antibody_FC_2_021815
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
149603 25 µg £109
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149604 100 µg £254
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Description

CD182, also known as CXCR2, is a G-protein coupled receptor with seven transmembrane regions that is involved in chemotaxis, neutrophil activation, and angiogenesis. CXCR2 is expressed by neutrophils, basophils, a subset of T cells, monocytes, macrophages, natural killer (NK) and natural killer T cells (NKT). Its ligands are CXCL1, CXCL2, CXCL3, and CXCL5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CXCR2-transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  2. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  3. Del Prete A, et al. 2017. Blood. 130:1223. PubMed
  4. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  5. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  6. Dave M, et al. 2016. Infect Immun . 84: 3172 - 3181. PubMed
RRID
AB_2565562 (BioLegend Cat. No. 149603)
AB_2565562 (BioLegend Cat. No. 149604)

Antigen Details

Structure
G-protein coupled receptor and seven transmembrane regions.
Distribution

Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer (NK) cells.

Function
Chemotaxis, neutrophil activation, and angiogenesis.
Ligand/Receptor
CXCL1, CXCL2, CXCL3, and CXCL5.
Cell Type
Basophils, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Spaan AN, et al. 2014. Nat. Commun. 5:5438.
2. Natsuaki Y, et al. 2014. Nat. Immunol. 15:1064.
3. Hsieh CY, et al. 2014. J. Immunol. 193:3693.
4. Diana J and Lehuen A. 2014. EMBO Mol. Med. 6:1090.
5. Stoolman JS, et al. 2014. J. Immunol. 193:564.

Gene ID
12765 View all products for this Gene ID
UniProt
View information about CD182 on UniProt.org
Go To Top Version: 1    Revision Date: 02/18/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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