Alexa Fluor 647 anti-mouse CD272 BTLA Antibody anti-CD272

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Clone: 6A6 (See other available formats)
Regulatory Status: RUO
Other Names: BTLA, B and T lymphocyte attenuator
Isotype: Armenian Hamster IgG
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Product Citations: publications

6A6_AF647_061510 — C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) PE and 6A6 Alexa Fluor® 647

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139106

100 µg

£221

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Description

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8⁺ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4⁺ and CD8⁺ T cells in vivo. Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm. The existence of three distinct BTLA alleles has been reported.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: C57BL/6 BTLA Ig domain protein in CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation^1,2 and blocking^3,5 of OT-I T cell responses in vitro and incidence of cerebral malaria in vivo caused by P. berghei ANKA (PbA) infection. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 139104). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 139117 - 139122) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Clone 6A6 antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.
RRID: AB_10613297 (BioLegend Cat. No. 139106)

Antigen Details

Structure: An Ig superfamily co-inhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution: BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4⁺ T cells, lower expression on CD8⁺ T cells, macrophages, dendritic cells, NKT cells, and NK cells.
Function: BTLA functions as a negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
Ligand/Receptor: HVEM.
Cell Type: B cells, Dendritic cells, Lymphocytes, NKT cells, T cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Liu X, et al. 2009. J Immunol. 182:4516.
2. Miller ML, et al. 2009. J Immunol. 183:32.
3. Sun Y, et al. 2009. J Immunol. 183:1946.
4. Vendel AC, et al. 2009. J Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat Immunol. 4:670.
Gene ID: 208154 View all products for this Gene ID
UniProt: View information about CD272 on UniProt.org

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Other Formats

View All CD272 Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 647 anti-mouse CD272 (BTLA)	6A6	FC
PE anti-mouse CD272 (BTLA)	6A6	FC
APC anti-mouse CD272 (BTLA)	6A6	FC
TotalSeq™-A0881 anti-mouse CD272 (BTLA)	6A6	PG
PE/Cyanine7 anti-mouse CD272 (BTLA)	6A6	FC
Ultra-LEAF™ Purified anti-mouse CD272 (BTLA)	6A6	FC,IP
TotalSeq™-C0881 anti-mouse CD272 (BTLA)	6A6	PG
PE/Fire™ 640 anti-mouse CD272 (BTLA)	6A6	FC
PE/Fire™ 700 anti-mouse CD272 (BTLA)	6A6	FC
TotalSeq™-B0881 anti-mouse CD272 (BTLA)	6A6	PG

Certificate of Analysis

Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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