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Alexa Fluor® 647 anti-mouse CD309 (VEGFR2, Flk-1) Antibody

Pricing & Availability
Clone
89B3A5 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, fetal liver kinase-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
89B3A5_Alx647_021808
Mouse FLK-1 transfected cells stained with 89B3A5 Alexa Fluor® 647
  • 89B3A5_Alx647_021808
    Mouse FLK-1 transfected cells stained with 89B3A5 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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121909 25 µg £85
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121910 100 µg £182
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Description

The 89B3A5 antibody recognizes mouse CD309 also known as vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF or VEGFC; ligand binding plays a key role in vascular development and vascular permeability. The 89B3A5 antibody has been shown to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat-1 cells transfected with full-length mouse Flk
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kaburn N, et al. 1997. Development 124:2039.
Product Citations
  1. Verhoeven J, et al. 2022. Methods Mol Biol. 2572:45. PubMed
  2. Siatra P, et al. 2023. NPJ Regen Med. 8:13. PubMed
  3. Rignault-Clerc S, et al. 2013. Burns. 39:694. PubMed
  4. Glaser D, et al. 2016. PLoS One. 11:e0166663. PubMed
  5. Tanaka T, et al. 2012. Blood. 120:1299. PubMed
  6. N. H, et al. 2017. Journal of Vascular Research. 10.1159/000464287. PubMed
  7. Moschetta M, et al. 2016. Leukemia. 10.1038/leu.2016.3. PubMed
  8. Rüder C, et al. 2014. PLoS One. 9:104644. PubMed
  9. Hamerlik P, et al. 2012. J Exp Med. 209:507. PubMed
  10. Wu Q, et al. 2013. Eur J Pharmacol. 707:87. PubMed
  11. Xie X, et al. 2018. FASEB J. 32:1296. PubMed
RRID
AB_756169 (BioLegend Cat. No. 121909)
AB_756169 (BioLegend Cat. No. 121910)

Antigen Details

Structure
Member of the tyrosine protein kinase family, sub-family CSF-1/PDGF. Single pass transmembrane receptor containing a protein kinase domain and seven immunoglobulin-like domains.
Distribution

Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels.

Function
Receptor for VEGF or VEGFC. Ligand binding plays a key role in vascular development and vascular permeability.
Ligand/Receptor
VEGF, VEGFC
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Patterson C, et al. 1995. J. Biol. Chem. 270:23111.
2. Quinn TP, et al. 1993. Proc. Natl. Acad. Sci. USA 90:7533.

Gene ID
16542 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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