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Alexa Fluor® 647 anti-mouse CD317 (BST2, PDCA-1) Antibody

Pricing & Availability
Clone
129C1 (See other available formats)
Regulatory Status
RUO
Other Names
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_129C1_A647_020210
C57BL/6 mouse splenocytes stained with 129C1 Alexa Fluor® 647 and CD45R/B220 (RA3-6B2) PE
  • A_129C1_A647_020210
    C57BL/6 mouse splenocytes stained with 129C1 Alexa Fluor® 647 and CD45R/B220 (RA3-6B2) PE
  • B_129C1_A647_020210
    C57BL/6 mouse splenocytes stained with rat IgG2b Alexa Fluor® 647 isotype control and CD45R/B220 (RA3-6B2) PE
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127105 25 µg £97
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127106 100 µg £201
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Description

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 was recently identified as an IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse plasmacytoid dendritic cells (DCs)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Blasius AL, et al. 2006. J. Immunol. 177:3260.
Product Citations
  1. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  2. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  3. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
RRID
AB_2228222 (BioLegend Cat. No. 127105)
AB_2228222 (BioLegend Cat. No. 127106)

Antigen Details

Structure
Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution
Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
Function
Recently identified as antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type
Dendritic cells, B cells
Biology Area
Immunology, Innate Immunity, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Douglas JL, et al. 2009. J Virol. 83:7931.
2. Cao W, et al. 2009. J. Exp. Med. 206:1603.
3. Neil SJ, et al. 2008. Nature 451:425.

Gene ID
69550 View all products for this Gene ID
UniProt
View information about CD317 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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