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Alexa Fluor® 647 anti-mouse CLEC4F Antibody

Pricing & Availability
Clone
3E3F9 (See other available formats)
Regulatory Status
RUO
Other Names
C-Type Lectin Domain Family 4 Member F (CLEC4F), C-Type Lectin, Superfamily Member 13 (CLECSF13), Kupffer cell receptor (KCR), KCLR
Isotype
Mouse IgG1, κ
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Product Citations
publications
3E3F9_A647_CLEC4F_Antibody_082919.png
C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-mouse CLEC4F (clone 3E3F9) Alexa Fluor® 647 (red) and 10 µg/mL of anti-mouse CD105 (clone MJ7/18) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 20X objective.
  • 3E3F9_A647_CLEC4F_Antibody_082919.png
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-mouse CLEC4F (clone 3E3F9) Alexa Fluor® 647 (red) and 10 µg/mL of anti-mouse CD105 (clone MJ7/18) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 20X objective.
  • 3E3F9_A647_CLEC4F_Antibody_3DIHC_052621.png
    Paraformaldehyde-fixed (1%), 500 μm-thick mouse liver tissue section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD324 (E-Cadherin) antibody (clone DECMA-1) Alexa Fluor® 594 at 5 μg/mL (yellow), and anti-mouse CLEC4F antibody (clone 3E3F9) Alexa Fluor® 647 at 5 μg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 647 spectral data
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156803 25 µg £101
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156804 100 µg £249
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Description

CLEC4F, also known as Kupffer cell receptor or KCR, is an 80 kDa type II transmembrane glycoprotein of the C-type lectin superfamily. It is expressed on resident Kupffer cells and infiltrating monocytes in the liver but not on macrophages in other tissues. CLEC4F interacts with alpha-galactosylceramide (α-GalCer) in a calcium-dependent manner and participates in the presentation of α-GalCer to natural killer T (NKT) cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Zeng J, et al. 2023. Nat Commun. 14:2573. PubMed
  2. Shang Y, et al. 2022. Br J Pharmacol. 179:4378. PubMed
  3. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  4. Glennon EKK, et al. 2022. Front Cell Infect Microbiol. 11:804186. PubMed
  5. Lam P, et al. 2022. Mol Ther Methods Clin Dev. 26:413. PubMed
  6. Cho CS, et al. 2021. Cell. 184(13):3559-3572.e22. PubMed
RRID
AB_2814081 (BioLegend Cat. No. 156803)
AB_2814081 (BioLegend Cat. No. 156804)

Antigen Details

Structure
Type II transmembrane glycoprotein
Distribution

Kupffer cells

Function
Modulates glycolipids presentation on Kupffer cells.
Ligand/Receptor
Fucose, Galactose, N-acetylgalactosamine, un-sialylated glucosphingolipids with GalNAc or Gal terminus
Antigen References
  1. Yang CY, et al. 2013. PLoS One. 8(6):e65070.
  2. van der Tuin SJL, et al. 2018. J Am Heart Assoc. 7(6)
  3. Hsieh SL, et al. 2009. J. Immunol. 182:78.38.
Gene ID
51811 View all products for this Gene ID
UniProt
View information about CLEC4F on UniProt.org
Go To Top Version: 3    Revision Date: 05/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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