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Alexa Fluor® 647 anti-mouse FOXP3 Recombinant Antibody

Pricing & Availability
Clone
QA20A67 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A67_A647_Antibody_FOXP3_011224
C57BL/6 mouse splenocytes were surfaced stained with anti-mouse CD4 (clone GK1.5) PE. Cells were fixed and permeabilized with True Nuclea™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-mouse FOXP3 recombinant (clone QA20A67) Alexa Fluor® 647 (left) or rat IgG1, κ Alexa Fluor® 647 isotype control (right).
  • QA20A67_A647_Antibody_FOXP3_011224
    C57BL/6 mouse splenocytes were surfaced stained with anti-mouse CD4 (clone GK1.5) PE. Cells were fixed and permeabilized with True Nuclea™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-mouse FOXP3 recombinant (clone QA20A67) Alexa Fluor® 647 (left) or rat IgG1, κ Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
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118905 25 µg £134
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118906 100 µg £257
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Description

FOXP3, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX, is a 47 kD transcription factor. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone QA20A67 can completely block the binding of clone MF-14 on target cells.

RRID
AB_3097539 (BioLegend Cat. No. 118905)
AB_3097539 (BioLegend Cat. No. 118906)

Antigen Details

Structure
Forkhead/winged-helix transcription factor
Distribution

T regulatory cells

Function
Transcription Factor
Cell Type
Lymphocytes, T cells, Tregs
Biology Area
Immunology, Transcription Factors
Antigen References
  1. Ono M, et al. 2007. Nature. 446:685-9.
  2. Hori S, et al. 2003. Science. 299:1057-61.
  3. Fontenot JD, et al. 2003. Nat Immunol. 4:330-6.
Gene ID
20371 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 1    Revision Date: 01/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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