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Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
MC-480 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
Isotype
Mouse IgM, κ
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Product Citations
publications
MC-480_A647_081908.jpg
F9 (mouse embryonic carcinoma cell line) stained with MC-480 Alexa Fluor® 647
  • MC-480_A647_081908.jpg
    F9 (mouse embryonic carcinoma cell line) stained with MC-480 Alexa Fluor® 647
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125607 25 tests £113
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125608 100 tests £254
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Description

The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565. (IHC, IP, WB)
Product Citations
  1. Wu L, et al. 2021. Cell Prolif. 54:e13133. PubMed
  2. Khodeer S, et al. 2022. J Cell Sci. 135: . PubMed
  3. Zhang J, et al. 2018. Nature. 562:595. PubMed
  4. Lynch TJ et al. 2018. Cell stem cell. 22(5):653-667 . PubMed
  5. Kim S, et al. 2015. Cancer Lett . Available online 29 August 2015. PubMed
  6. Mitry MA, et al. 2020. American Journal of Physiology-Cell Physiology. 318(2):C380-C391. PubMed
  7. Chen J, et al. 2016. Cell Rep. S2211-1247(16)00033-4. PubMed
  8. Deb S, et al. 2021. EMBO Rep. 22:e49328. PubMed
  9. Yu S, et al. 2022. Nat Commun. 13:2756. PubMed
  10. Borkent M, et al. 2016. Stem Cell Reports. 6: 704-716. PubMed
  11. Hill RJ, et al. 2019. Nat Genet. 51:1283. PubMed
  12. Vojtek M, et al. 2021. Life Sci Alliance. 4:. PubMed
  13. Fujino N, et al. 2019. J Exp Med. 216:2184. PubMed
RRID
AB_1089190 (BioLegend Cat. No. 125607)
AB_1089190 (BioLegend Cat. No. 125608)

Antigen Details

Structure
Carbohydrate epitope, Lewis blood group antigens
Distribution

Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells

Function
Adhesion, differentiation
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
2. Harris JF, et al. 1984. J. Immunol. 132:2502.
3. Gooi HC, et al. 1981. Nature 292:156.
4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447.

Gene ID
14345 View all products for this Gene ID 2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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