Alexa Fluor 647 anti-mouse IL-17F Antibody anti-IL-17F

Pricing & Availability

Clone: 9D3.1C8 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-17F, ML-1,
Isotype: Mouse IgG1, κ
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9D3.1C8_AF647_1_060710 — PdBU/ionomycin-stimulated (5 hours) Th17-polarized CD4⁺ T cells from C57BL/6 mouse lymph nodes surface stained with CD4 (GK1.5) PE, then intracellularly stained with 9D3.1C8 Alexa Fluor® 647

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517003

25 µg

£121

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517004

100 µg

£249

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Description

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member. The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F. IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells. IL-17F is an important regulator of inflammatory responses. It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. Over expression of IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hyper-secretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo. P38 MAPK, ERK1/2, Act1 (NF-_KB activator protein 1), and TRAF6 are involved in IL-17F signaling.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse IL-17F-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2249030 (BioLegend Cat. No. 517003)
AB_2249030 (BioLegend Cat. No. 517004)

Antigen Details

Structure: Forms a 37 kD homodimer or heterodimer with IL-17A of the IL-17 family member.
Distribution: Th17 cells, mast cells, basophils, and epithelial cells.
Function: An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand/Receptor: IL-17R (CD217)
Cell Type: Th17, Mast cells, Basophils, Epithelial cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Dong C. 2008. Immunol. Rev. 226:80.
2. Kolls JK, et al. 2004. Immunity 21:467.
3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1.
4. Yang XO, et al. 2008. J. Exp. Med. 205:1063.
5. Ishigame H, et al. 2009. Immunity 30:108.
6. Hu Y, et al. 2010. J. Immunol. 184:4307.
Gene ID: 257630 View all products for this Gene ID
UniProt: View information about IL-17F on UniProt.org

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Other Formats

View All IL-17F Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 488 anti-mouse IL-17F	9D3.1C8	ICFC
PE anti-mouse IL-17F	9D3.1C8	ICFC
Alexa Fluor® 647 anti-mouse IL-17F	9D3.1C8	ICFC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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