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Alexa Fluor® 647 anti-mouse IL-17F Antibody

Pricing & Availability
Clone
9D3.1C8 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17F, ML-1,
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9D3.1C8_AF647_1_060710
PdBU/ionomycin-stimulated (5 hours) Th17-polarized CD4+ T cells from C57BL/6 mouse lymph nodes surface stained with CD4 (GK1.5) PE, then intracellularly stained with 9D3.1C8 Alexa Fluor® 647
  • 9D3.1C8_AF647_1_060710
    PdBU/ionomycin-stimulated (5 hours) Th17-polarized CD4+ T cells from C57BL/6 mouse lymph nodes surface stained with CD4 (GK1.5) PE, then intracellularly stained with 9D3.1C8 Alexa Fluor® 647
  • 9D3.1C8_AF647_2_060710
    PdBU/ionomycin-stimulated (5 hours) Th17-polarized CD4+ T cells from C57BL/6 mouse lymph nodes intracellularly stained with IL-17A (TC11-18H10.1) PE and 9D3.1C8 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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517003 25 µg £121
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517004 100 µg £249
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Description

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Over expression of IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hyper-secretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo.  P38 MAPK, ERK1/2, Act1 (NF-KB activator protein 1), and TRAF6 are involved in IL-17F signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-17F-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. Watanabe N, et al. 2016. PLoS One. 11: 0150244. PubMed
  3. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  4. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  5. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
RRID
AB_2249030 (BioLegend Cat. No. 517003)
AB_2249030 (BioLegend Cat. No. 517004)

Antigen Details

Structure
Forms a 37 kD homodimer or heterodimer with IL-17A of the IL-17 family member.
Distribution
Th17 cells, mast cells, basophils, and epithelial cells.
Function
An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand/Receptor
IL-17R (CD217)
Cell Type
Th17, Mast cells, Basophils, Epithelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dong C. 2008. Immunol. Rev. 226:80.
2. Kolls JK, et al. 2004. Immunity 21:467.
3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1.
4. Yang XO, et al. 2008. J. Exp. Med. 205:1063.
5. Ishigame H, et al. 2009. Immunity 30:108.
6. Hu Y, et al. 2010. J. Immunol. 184:4307.

Gene ID
257630 View all products for this Gene ID
UniProt
View information about IL-17F on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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