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Alexa Fluor® 647 anti-mouse Ly-51 Antibody

Pricing & Availability
Clone
6C3 (See other available formats)
Regulatory Status
RUO
Other Names
6C3/BP-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
6C3_Alx647_A_020708
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
  • 6C3_Alx647_A_020708
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
  • 6C3_Alx647_B_020708
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
Compare all formats See Alexa Fluor® 647 spectral data
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108311 25 µg £70
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108312 100 µg £157
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Description

Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
  2. Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
  3. Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
  4. Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
  5. Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
  6. Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
  7. Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
  8. Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
Product Citations
  1. Miyao T, et al. 2022. Elife. 11: . PubMed
  2. Wang F, et al. 2024. Nat Commun. 203:15. PubMed
  3. Fujimori S, et al. 2022. Elife. 11:. PubMed
  4. Ohigashi I, et al. 2021. Cell Rep. 36:109657. PubMed
  5. Dumont-Lagacé M, et al. 2014. J Immunol. 192:2219. PubMed
  6. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  7. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  8. Gruver A, et al. 2009. J Endocrinol. 203:75. PubMed
  9. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  10. Gulati GS, et al. 2018. Nat Protoc. 13:1294. PubMed
  11. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  12. Chen P, et al. 2017. Sci Rep. . 10.1038/s41598-017-16162-2. PubMed
  13. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  14. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  15. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  16. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  17. St-Pierre C, et al. 2015. J Immunol. 195: 498 - 506. PubMed
  18. Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed
  19. Menon S, et al. 2021. Nat Commun. 12:4640. PubMed
  20. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  21. Dumont-Lagacé M, et al. 2015. Sci Rep. 5: 12895. PubMed
  22. Liang Z, et al. 2022. iScience. 25:105233. PubMed
RRID
AB_2099613 (BioLegend Cat. No. 108311)
AB_2099613 (BioLegend Cat. No. 108312)

Antigen Details

Structure
Aminopeptidase A, 140 kD
Distribution

B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells

Function
Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
Cell Type
B cells, Dendritic cells, Epithelial cells
Biology Area
Immunology
Antigen References

1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681.

Gene ID
13809 View all products for this Gene ID
UniProt
View information about Ly-51 on UniProt.org
Go To Top Version: 4    Revision Date: 10/10/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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