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Alexa Fluor® 647 anti-mouse RAE-1γ Antibody

Pricing & Availability
Clone
CX1 (See other available formats)
Regulatory Status
RUO
Other Names
Retinoid acid early inducible gamma
Isotype
Rat IgG2b, κ
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Product Citations
publications
CX1_A647_052209.jpg
YAC-1 cells stained with CX1 Alexa Fluor® 647
  • CX1_A647_052209.jpg
    YAC-1 cells stained with CX1 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
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130110 100 µg £157
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Description

RAE-1 gamma is one of the five member RAE-family.Together with H60 they were identified as ligands for the mouse NKG2D molecules.Expression of RAE-1 is low or absent in normal adult tissue, but can be upreulgated by retinoid acid and constitutively expressed on some tumors.This antibody has weak crossreativity with RAE-1 alpha and RAE-1 beta.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse RAE-1 gamma transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Lodoen M, et al. 2003. J. Exp. Med. 197:1245.
RRID
AB_2175361 (BioLegend Cat. No. 130110)
Disclaimer

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Antigen Details

Structure
RAE is a family of GPI-anchord surface protein consisting of alpha, beta, gamma, delta and epsilon. They share strong homology within the family and weak homology with MHC class 1 molecule.
Distribution

Expression of RAE-1 is low or absent on normal, adult tissue, but consititutively expressed on some tumors and can be induced by retinoid acid.

Function
As ligands for the mouse activating NKG2D expressed by NK cells
Ligand/Receptor
NKG2D
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Cerwenka A, et al. 2000. Immnity. 12:721
2. Lodoen M, et al. 2003 J. Exp. Med. 197:1245
3. Diefenbach A, et al. 2001 Nature 413:165

Gene ID
66679 View all products for this Gene ID
UniProt
View information about RAE-1gamma on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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