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- Clone
- F31-5G3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell immunoglobulin and mucin domain containing protein-4
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Balb/c mouse peritoneal macrophages stained with F31-5G3 Alexa Fluor® 647 and CD11b (M1/70) FITC
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 129907 | 25 µg | £101 | ||||
TIM4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells.TIM4 binds to TIM1 to promote T cell proliferation by enhancing cell division and reducing apoptosis.TIM4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse TIM4-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706
- RRID
-
AB_1227797 (BioLegend Cat. No. 129907)
Antigen Details
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain
- Distribution
-
Unlike the other TIMs, TIM4 is only expressed by antigen-presenting cells, but not by T cells.
- Function
- TIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
- Interaction
- Phosphatiylserine is the ligand of TIM4 and TIM4 is a ligand of TIM1
- Ligand/Receptor
- Phosphatidylserine, TIM1
- Cell Type
- Antigen-presenting cells
- Biology Area
- Immunology
- Antigen References
-
1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R et al. 2008. J. Immunol. 180:4706 - Gene ID
- 276891 View all products for this Gene ID
- UniProt
- View information about Tim-4 on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All Tim-4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse Tim-4 | F31-5G3 | FC |
| Alexa Fluor® 647 anti-mouse Tim-4 | F31-5G3 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-mouse Tim-4
BALB/c peritoneal macrophages stained with F31-5G3 PE and M1... -
Alexa Fluor® 647 anti-mouse Tim-4
Balb/c mouse peritoneal macrophages stained with F31-5G3 Ale...
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