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Alexa Fluor® 647 anti-Nestin Antibody

Pricing & Availability
Clone
Rat-401 (See other available formats)
Regulatory Status
RUO
Other Names
Nestin, NES
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Rat-401_A647_Nestin_Antibody_IHC-P_032818
IHC staining of Alexa Fluor® 647 anti-Nestin antibody (clone Rat-401) on formalin-fixed paraffin-embedded Embryonic Day 15 mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody 2 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • Rat-401_A647_Nestin_Antibody_IHC-P_032818
    IHC staining of Alexa Fluor® 647 anti-Nestin antibody (clone Rat-401) on formalin-fixed paraffin-embedded Embryonic Day 15 mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody 2 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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655107 25 µg £109
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655108 100 µg £245
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Description

Nestin was originally identified as a neuronal stem cell marker during central nervous system (CNS) development. It belongs to an intermediate filament protein family, and it is the most widely used marker for identifying neuronal stem cells. Nestin is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. However, the exact function of nestin is not very clear. Nestin is involved in structural organization of the cells through assembling into heterodimers with vimentin or α-internexin in a phosphorylation-dependent process to form intermediate filaments. The expression of nestin is downregulated and replaced by expression of the neurofilament proteins upon neural differentiation/maturation.

Expression of nestin has also been reported in non-neuronal immature or progenitor cells such as pancreatic islet progenitor cells, angiogenic endothelial cells, glioma cells, and bone marrow mesenchymal stem cells. Recent studies also showed that nestin expression can be detected in repair processes in the CNS and in various tumor cells. Thus, it has been used as a cancer stem cell marker. The usage of nestin as a novel prognostic indicator and possible target for tumor anigogenesis has been also proposed.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Homogenized Sprague-Dawley rat spinal cord.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Tissue specificity of this Alexa Fluor® 647 anti-Nestin antibody was validated in embryonic day 15 mouse brain tissue.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The reactivity of Alexa Fluor® 647 anti-Nestin antibody was verified in FFPE embryonic mouse tissue.

Additional reported applications (for the relevant formats) include: immunofluorescence, immunohistochemistry, and immunoprecipitation1-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Hockfield S, et al. 1985. J. Neurosci. 5:3310. (IP)
  2. Friedman B, et al. 1990. J. Comp. Neurol. 295:43. (IP)
  3. Brook GA, et al. 1994. Exp. Neurol. 126:31. (IP)
  4. Lendahl U, et al. 1990. Cell 60:585. (IP)
Product Citations
  1. Feng H, et al. 2020. J Clin Invest. 130:6354. PubMed
  2. Ge H, et al. 2021. Front Mol Neurosci. 13:596039. PubMed
RRID
AB_2728489 (BioLegend Cat. No. 655107)
AB_2728489 (BioLegend Cat. No. 655108)

Antigen Details

Structure
Mouse nestin is 1864 amino acids with a predicted molecular weight of 207 kD. The observed molecular weight is larger than 250 kD.
Distribution

Cytosol

Function
Nestin gene encodes a member of the intermediate filament, and it is required for survival and renewal of neural progenitor cells.
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Lendahl U, et al. 1990. Cell 60:585.
2. Gilyarov AV, et al. 2008. Neurosci. Behav. Physiol. 38:165.
3. Méndez-Ferrer S, et al. 2010. Nature 466:829.
4. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.

Gene ID
10763 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org
Go To Top Version: 1    Revision Date: 03/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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