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Alexa Fluor® 647 anti-Oligodendrocyte Marker O1 Antibody

Pricing & Availability
Clone
O1 (See other available formats)
Regulatory Status
RUO
Other Names
Galactocerebroside O1, Oligodendrocyte marker O1, Oligodendrocyte O1 antigen
Isotype
Mouse IgM, κ
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Product Citations
publications
A_O1_A647_Oligodendrocyte_IHC_082923
Formalin-fixed paraffin-embedded human cerebellum tissue was stained with Alexa Fluor® 647 anti-Oligodendrocyte Marker O1 (clone O1). Following antigen retrieval using Citrate buffer (Cat. No. 420902), the tissue was incubated without (panel A) or with (panels B, C, and D) 1.0 μg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective (panels A and B) and a 40X objective (panels C and D). Scale bar: 50 μm
  • A_O1_A647_Oligodendrocyte_IHC_082923
    Formalin-fixed paraffin-embedded human cerebellum tissue was stained with Alexa Fluor® 647 anti-Oligodendrocyte Marker O1 (clone O1). Following antigen retrieval using Citrate buffer (Cat. No. 420902), the tissue was incubated without (panel A) or with (panels B, C, and D) 1.0 μg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective (panels A and B) and a 40X objective (panels C and D). Scale bar: 50 μm
  • B_O1_A647_Oligodendrocyte_ICC_082923
    Differentiated rat cortical stem cells were fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained without (panel A) or with (panel B) 10 µg/mL of Alexa Fluor® 647 anti-Oligodendrocyte Marker O1 (clone O1) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 μm
  • C_O1_A647_Oligodendrocyte_ICFC_082923
    Differentiated rat cortical stem cells were fixed and permeabilized using Cyto-Fast Buffer (Cat. No. 426803) and stained with Alexa Fluor® 647 anti-Oligodendrocyte Marker O1 (clone O1) (filled histogram), or Alexa Fluor® 647 Mouse IgM, κ isotype control (open histogram, dashed line) (Cat No. 401618).
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607757 25 µg £148
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607758 100 µg £264
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Description

Oligodendrocytes are the myelinating cells of the central nervous system (CNS). Similar to the Schwann cells in the peripheral nervous system (PNS), their function is to provide support and isolation to neuronal axons. Their importance has been denoted on de-myelinating diseases such as multiple sclerosis, amyotrophic lateral sclerosis, periventricular leukomalacia, and also, they have been associated with dementia, schizophrenia, cognitive impairment, and bipolar disorder. Oligodendrocytes develop during embryogenesis; however, a small proportion of progenitor stem cells are present in the adult brain. Thus, three stages of differentiation can be identified in the cerebral white matter, and specific markers have been associated which each particular stage. Galactocerebroside O1 (marker O1) is one of the major galactosphingolipids components of oligodendrocytes plasma membrane. It is produced from late immature non-myelinating oligodendrocytes to mature myelinating oligodendrocytes, and together with expression of CNPase, it has been widely used as characteristic marker for these specific differentiation stages.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
White matter from bovine corpus callosum (from developer)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on testis samples, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemistry on cerebellum samples, a concentration range of 0.5 - 1.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone O1 recognize specifically O1 antigen by immunocytochemistry in oligodendrocytes derived from cortical stem cell using different fixation/permeabilization methods such as methanol, PFA/Triton X-100, and PFA/methanol, where the last method shows the highest intensity.

By immunohistochemistry in paraffin sections, clone O1 showed specific high staining on cerebellum white matter, and not staining in the molecular layer at the recommended concentration.

By flow cytometry staining on differentiated cortical stem cells, clone O1 stained at high intensity complex cells (oligodendrocytes) compared with low complexity cells (undifferentiated progenitor stem cells).

RRID
AB_3068297 (BioLegend Cat. No. 607757)
AB_3068297 (BioLegend Cat. No. 607758)

Antigen Details

Structure
It is a galactocerebroside (glycosphingolipid), composed of a single galactose linked to a ceramide
Distribution

Present in the plasma membrane of late immature to mature oligodendrocytes. 

Cell Type
Neural Stem Cells, Oligodendrocytes
Biology Area
Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Bansal R, et al. 1989. J Neurosci Res. 24:548-57.
  2. Bansal R, et al. 1999. J Neurosci. 19:7913-24.
  3. Ahmad I, et al. 2021. PloS One. 16:e0256155.
  4. Rasti BF, et al. 2020. Int J Nanomedicine. 15:3903-3920.
Gene ID
NA
UniProt
View information about Oligodendrocyte Marker O1 on UniProt.org

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Go To Top Version: 1    Revision Date: 08/29/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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