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Alexa Fluor® 647 anti-Puromycin Antibody

Pricing & Availability
Clone
2A4 (See other available formats)
Regulatory Status
RUO
Other Names
Puromycin from streptomyces alboniger
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2A4_AF647_Puromycin_Antibody_050222
The human T-lymphoblast cell line, MOLT-4, was treated with Puromycin (1 µg/mL) for 1h (filled histogram) or left untreated (open histogram) and then intracellularly stained with anti-Puromycin (clone 2A4) Alexa Fluor® 647 using the Cyto-Fast™ Fix/Perm Buffer Set.
  • 2A4_AF647_Puromycin_Antibody_050222
    The human T-lymphoblast cell line, MOLT-4, was treated with Puromycin (1 µg/mL) for 1h (filled histogram) or left untreated (open histogram) and then intracellularly stained with anti-Puromycin (clone 2A4) Alexa Fluor® 647 using the Cyto-Fast™ Fix/Perm Buffer Set.
Compare all formats See Alexa Fluor® 647 spectral data
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381507 25 tests £129
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381508 100 tests £273
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Description

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Puromycin from Streptomyces alboniger coupled to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase.

For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody.  

RRID
AB_2927826 (BioLegend Cat. No. 381507)
AB_2927826 (BioLegend Cat. No. 381508)

Antigen Details

Distribution

Cytoplasm, Ribosomes

Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Protein Synthesis
Antigen References
  1. Schmidt E, et al. 2009. Nat Methods. 6:275-7.
  2. Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075.
Gene ID
NA
UniProt
View information about Puromycin on UniProt.org
Go To Top Version: 1    Revision Date: 05/02/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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