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Alexa Fluor® 647 anti-rat CD45RC Antibody

Pricing & Availability
Clone
OX-22 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-22_A647_011909.jpg
LOU rat splenocytes stained with ox-22 Alexa Fluor® 647
  • OX-22_A647_011909.jpg
    LOU rat splenocytes stained with ox-22 Alexa Fluor® 647
  • OX-22_A647_CD45RC_Antibody_2_010419
    Rat frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD4 (clone W3/25) Alexa Fluor® 488 (blue), 10 µg/mL of CD45RC (clone OX-22) Alexa Fluor® 647 (yellow), and 10 µg/mL of purified CD29 (HMβ1-1) overnight at 4°C, followed by 5 µg/mL of anti-Armenian hamster IgG (clone Poly4055) Alexa Fluor® 594 (red) for two hours at room temperature. The image was captured by 10X objective.
See Alexa Fluor® 647 spectral data
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204010 100 µg £182
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Description

CD45RC is a 200-220 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant (exon C) of the tyrosine phosphatase CD45. The CD45RC isoform is expressed on B cells, majority of CD8+ T cells and a subset of CD4+ T cells, but not on myeloid cells. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens, including CD1, CD2, CD3, and CD4. CD45 is involved in signaling lymphocytes activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA-activated rat lymph node cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2, 3 and formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Woollett GR, et al. Eur. J. Immunol. 15:168.
  2. Spickett GP, et al. 1983. J. Exp. Med. 158:795.
  3. Mason DW, et al. 1983. Immunol. Rev. 74:57.
Product Citations
  1. Dragovich MA, et al. 2019. PLoS One. e0218109:14. PubMed
RRID
AB_2174547 (BioLegend Cat. No. 204010)

Antigen Details

Cell Type
B cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Gene ID
544392 View all products for this Gene ID 544377 View all products for this Gene ID
UniProt
View information about CD45RC on UniProt.org
Go To Top Version: 3    Revision Date: 01/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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