Alexa Fluor® 647 anti-Synaptophysin Antibody, Synaptophysin, SP17

Pricing & Availability

Clone: SP17 (See other available formats)
Regulatory Status: RUO
Other Names: SYP, Synaptophysin, Major synaptic vesicle protein P38
Isotype: Mouse IgM, κ
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Product Citations: publications

SP17_A-647_Synaptophysin_IHC-P_062524 — IHC staining of Alexa Fluor® 647 anti-Synaptophysin (clone SP17) on formalin-fixed paraffin-embedded human brain. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was then incubated with (panels A and B) and without (panels C and D) Alexa Fluor® 647 anti-Synaptophysin (clone SP17). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panels A and C) or a 40X (panels B and D) objective. Scale bar: 50 µm

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837105

25 µg

£124

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837106

100 µg

£308

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Description

Synaptophysin, also known as major synaptic vesicle protein P38, is encoded by the SYP gene in humans and is located on the short arm of the X chromosome. Synaptophysin is expressed in neuroendocrine cells as well as all neurons in the CNS that participate in synaptic transmission. Synaptophysin is a synaptic vesicle glycoprotein, and interacts with synaptobrevin. It has been implicated in X-linked mental retardation and can be used as a specific marker for cells of the adrenal medulla and pancreatic islets. As such, it can be used to identify tumors that are derived from those cells, including neuroblastoma, retinoblastoma, medulloblastoma, and others.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against a crude synaptic preparation from post mortem human brain.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: SP17 reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells. The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.
RRID: AB_3662339 (BioLegend Cat. No. 837105)
AB_3662339 (BioLegend Cat. No. 837106)

Antigen Details

Structure: Synaptophysin is a 313 amino acid protein with an apparent molecular mass of 34 kD.
Distribution: Tissue distribution: Brain, pancreas, adipose and soft tissue, gastrointestinal tract, bone marrow, and immune system.
Cellular Distribution: Plasma and synaptic vesicle membrane.
Function: Synaptophysin plays a role in synaptic vesicle docking and membrane fusion.
Interaction: SRCIN1, also interact with itself to form homohexamer or homotetramer
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Presynaptic proteins
Antigen References: Zhang L, et al. 2018. Medicine (Baltimore). 97(29):e11487

Moris, et al. 2018. Anticancer res. 38(2):601

Hami J, et al. 2017. J pediatr Neurosci. 12(3):215
Gene ID: 6855 View all products for this Gene ID
UniProt: View information about Synaptophysin on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All Synaptophysin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Synaptophysin	SP17	WB,ICC,IHC-P,SB
Alexa Fluor® 647 anti-Synaptophysin	SP17	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/25/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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