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Alexa Fluor® 700 anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI30_Alx700_111407
Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 700
  • HI30_Alx700_111407
    Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
304023 25 µg £85
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304024 100 µg £182
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ivarsson M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.50. PubMed
  2. Calabrese DR, et al. 2020. J Clin Invest. . PubMed
  3. Pal R, et al. 2020. Curr Protoc Cytom. 92:e69. PubMed
  4. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  5. Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed
  6. Filipello F, et al. 2023. Acta Neuropathol. 145:749. PubMed
  7. Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed
  8. Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
  9. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  10. Takano T, et al. 2022. Cell Rep Med. 3:100631. PubMed
  11. Bessy T, et al. 2021. J Cell Biol. 220:. PubMed
  12. Wu Y, et al. 2013. J Biosci Bioeng. 64:169. PubMed
  13. Nilsson A, et al. 2016. PLoS One. 11: 0158369. PubMed
  14. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  15. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  16. Fontana D, et al. 2020. Hemasphere. e497:4. PubMed
  17. Zheng S, et al. 2018. Mol Syst Biol. 14:e8041. PubMed
  18. Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed
  19. Perez–Zsolt D, et al. 2019. Front Immunol. 0.989583333. PubMed
  20. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
  21. Li L, et al. 2020. J Transl Med. 18:363. PubMed
  22. Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed
  23. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  24. Fedyk ER, et al. 2020. British Journal of Clinical Pharmacology. 86(7):1314-1325.. PubMed
  25. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  26. Marquardt N, et al. 2016. J Immunol. 197: 3069 - 3075. PubMed
  27. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  28. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  29. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  30. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  31. Neal JT, et al. 2018. Cell. 175:1972. PubMed
  32. Butch ER, et al. 2020. Cancer Research. 79(12):3112-3124.. PubMed
  33. Khatamzas E, et al. 2022. Nat Commun. 13:5586. PubMed
  34. Poon MML, et al. 2021. Cell Rep. 37:110071. PubMed
  35. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  36. Shen Z, et al. 2022. Immun Ageing. 19:55. PubMed
  37. Louka E, et al. 2021. Journal of Experimental Medicine. 218(2):. PubMed
  38. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  39. Hernndez DC, et al. 2021. Immunity. :. PubMed
  40. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  41. Strand K, et al. 2022. Front Endocrinol (Lausanne). 13:856530. PubMed
  42. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  43. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  44. Cartellieri M, et al. 2016. Blood Cancer J. 6:e458. PubMed
  45. Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
  46. Wu X, et al. 2020. Front Immunol. 1.922222222. PubMed
  47. Sun K, et al. 2022. Nat Commun. 13:4943. PubMed
  48. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  49. Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
  50. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  51. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  52. Williams DW, et al. 2021. Cell. . PubMed
  53. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
  54. Ludwig LS et al. 2019. Cell. 176(6):1325-1339 . PubMed
  55. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  56. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  57. Jühling F, et al. 2021. Life Sci Alliance. 4: . PubMed
RRID
AB_493760 (BioLegend Cat. No. 304023)
AB_493760 (BioLegend Cat. No. 304024)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD45 HI30 FC
Biotin anti-human CD45 HI30 FC
FITC anti-human CD45 HI30 FC
PE anti-human CD45 HI30 FC
PE/Cyanine5 anti-human CD45 HI30 FC
Purified anti-human CD45 HI30 FC,CyTOF®,IHC-P,WB,IHC-F,ICC
APC/Cyanine7 anti-human CD45 HI30 FC
PE/Cyanine7 anti-human CD45 HI30 FC
Alexa Fluor® 488 anti-human CD45 HI30 FC
Alexa Fluor® 647 anti-human CD45 HI30 FC
Pacific Blue™ anti-human CD45 HI30 FC
Alexa Fluor® 700 anti-human CD45 HI30 FC
PerCP anti-human CD45 HI30 FC
PerCP/Cyanine5.5 anti-human CD45 HI30 FC,SB
Brilliant Violet 421™ anti-human CD45 HI30 FC
Brilliant Violet 570™ anti-human CD45 HI30 FC
Brilliant Violet 510™ anti-human CD45 HI30 FC
Brilliant Violet 605™ anti-human CD45 HI30 FC
Brilliant Violet 650™ anti-human CD45 HI30 FC
Purified anti-human CD45 (Maxpar® Ready) HI30 FC,CyTOF®
Brilliant Violet 785™ anti-human CD45 HI30 FC
Brilliant Violet 711™ anti-human CD45 HI30 FC
PE/Dazzle™ 594 anti-human CD45 HI30 FC
Alexa Fluor® 594 anti-human CD45 HI30 IHC-P,SB
APC/Fire™ 750 anti-human CD45 HI30 FC
Pacific Blue™ anti-human CD45 HI30 FC
APC anti-human CD45 HI30 FC
PE/Cyanine7 anti-human CD45 HI30 FC
PE/Dazzle™ 594 anti-human CD45 HI30 FC
TotalSeq™-A0391 anti-human CD45 HI30 PG
TotalSeq™-B0391 anti-human CD45 HI30 PG
TotalSeq™-C0391 anti-human CD45 HI30 PG
PerCP/Cyanine5.5 anti-human CD45 HI30 FC
APC/Fire™ 750 anti-human CD45 HI30 FC
PE/Fire™ 640 anti-human CD45 HI30 FC
APC/Fire™ 810 anti-human CD45 HI30 FC
Spark YG™ 570 anti-human CD45 HI30 IHC-P
PE/Fire™ 700 anti-human CD45 HI30 FC
FITC anti-human CD45 HI30 FC
PerCP anti-human CD45 HI30 FC
Alexa Fluor® 660 anti-human CD45 Antibody HI30 FC
Spark Violet™ 538 anti-human CD45 HI30 FC
Spark YG™ 593 anti-human CD45 HI30 FC
GMP APC/Fire™ 750 anti-human CD45 HI30 FC
Spark Violet™ 538 anti-human CD45 HI30 FC
GMP APC anti-human CD45 HI30 FC
Spark UV™ 387 anti-human CD45 HI30 FC
GMP Pacific Blue™ anti-human CD45 HI30 FC
GMP PerCP anti-human CD45 HI30 FC
GMP FITC anti-human CD45 HI30 FC
PE anti-human CD45 HI30 FC
GMP PE/Dazzle™ 594 anti-human CD45 HI30 FC
GMP PerCP/Cyanine5.5 anti-human CD45 HI30 FC
Spark Blue™ 515 anti-human CD45 HI30 FC
TotalSeq™-D0391 anti-human CD45 HI30 PG
GMP PE/Cyanine7 anti-human CD45 HI30 FC
Spark Violet™ 500 anti-human CD45 HI30 FC
GMP PE anti-human CD45 HI30 FC
PE/Fire™ 810 anti-human CD45 HI30 FC
Spark PLUS UV395™ anti-human CD45 HI30 FC
Spark NIR™ 685 anti-human CD45 HI30 FC
Spark Violet™ 423 anti-human CD45 HI30 FC
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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