Alexa Fluor 700 anti-mouse Lineage Cocktail with Isotype Ctrl

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Clone: 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status: RUO
Other Names: Lin-1
Isotype: Rat IgG2a/Rat IgG2b
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Lineage_Cocktail_AF700_Antibody_FC_1_082112 — C57BL/6 mouse bone marrow cells were stained with CD117 FITC and Alexa Fluor® 700 lineage cocktail (top plot) or Alexa Fluor® 700 isotype controls (bottom plot).

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133313

25 tests

£201

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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Alexa Fluor® 700 Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Alexa Fluor® 700 Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
1. Alexa Fluor® 700 anti-mCD3/ Alexa Fluor® 700 anti-mLy-6G(Ly-6C)/ Alexa Fluor® 700 anti-mCD11b/ Alexa Fluor® 700 anti-mCD45R(B220)/ Alexa Fluor® 700 anti-mTer-119
2. Alexa Fluor® 700 Rat IgG2b/Rat IgG2a isotype controls

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Host Species: Rat
Formulation: This two-reagent set contains Alexa Fluor® 700 conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Storage & Handling: The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2715571 (BioLegend Cat. No. 133313)

Antigen Details

Distribution: T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes
Cell Type: B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Antigen References: 1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about Lineage on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Lineage Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-mouse Lineage Cocktail with Isotype Ctrl	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Pacific Blue™ anti-mouse Lineage Cocktail	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Brilliant Violet 421™ anti-mouse Lineage Cocktail	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC
Alexa Fluor® 700 anti-mouse Lineage Cocktail with Isotype Ctrl	17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70	FC

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 07/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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