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Alexa Fluor® 647 anti-EGR1 Antibody

Pricing & Availability
Clone
W16161B (See other available formats)
Regulatory Status
RUO
Other Names
Early Growth Response 1, ZNF225, NGFI-A, TIS8, AT225, G0S30, KROX-24, ZIF-268, Transcription Factor ETR103
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W16161B_Alexa-Fluor-647_EGR1_ICFC_041323
HeLa cells treated with 100 ng/mL recombinant human EGF (Cat. No. 585508) (positive control) (filled histogram) and untreated HeLa cells (negative control) (open histogram, solid line) were fixed and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-EGR1 (clone W16161B) or Alexa Fluor® 647 rat IgG2a, κ isotype control (Cat. No. 400526) (open histogram, dashed line).
  • a. W16161B_Alexa-Fluor-647_EGR1_ICFC_041323
    HeLa cells treated with 100 ng/mL recombinant human EGF (Cat. No. 585508) (positive control) (filled histogram) and untreated HeLa cells (negative control) (open histogram, solid line) were fixed and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-EGR1 (clone W16161B) or Alexa Fluor® 647 rat IgG2a, κ isotype control (Cat. No. 400526) (open histogram, dashed line).
  • b. W16161B_Alexa-Fluor-647_EGR1_ICC_041323.
    ICC staining of Alexa Fluor® 647 anti-EGR1 (clone W16161B) on HeLa cells treated for one hour with 100 ng/mL recombinant human EGF (Cat. No. 585508) (positive control) (lower panels), or untreated HeLa cells (negative control) (upper panels). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, blocked with 5% FBS for 1 hour at room temperature, and stained with Alexa Fluor® 647 anti-EGR1 (clone W16161B). Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 60X objective. Scale bar: 50 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
943903 25 tests £136
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943906 100 µg £266
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943904 100 tests £321
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Description

EGR1 (early growth response protein 1) is a zinc transcription factor vital to numerous physiological processes including cell cycle, differentiation, proliferation, metabolism, DNA damage response, and apoptosis. It also functions in mitogenesis, tissue repair, fibrosis, inflammatory response and immune response through its regulation of neutrophil gene expression and association with immune response genes such as TNFα, VEGF, and matrix metalloproteinases. In the central nervous system, EGR1 functions as an immediate early gene (IEG) and is vital to neuronal function and signaling, synaptic plasticity, cognition, and memory formation. Activated by acute mechanical injury and vascular stress, EGR1 regulates multiple cardiovascular pathological processes including atherosclerosis, intimal thickening and vascular repair in response to vascular injury, ischemia, angiogenesis, and cardiac hypertrophy. EGR1 is essential to the development, homeostasis, and repair of connective tissues through its regulation of genes of the extracellular matrix. EGR1 is a component of some oncogenic pathways and stimulates tumor growth and proliferation in prostate and gastric cancers. It also regulates a variety of tumor suppressors such as p53, PTEN, NAG-1, and TGFβ1, contributes to DNA repair, and induces apoptosis of tumor cells, thus functioning in dual roles as both an oncogene and as a tumor suppressor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human EGR1 protein
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC (test sizes) - Quality tested
ICC (µg size) - Quality tested
Recommended Usage

Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

When using this clone for ICFC, we recommend using the True Phos perm buffer. We do not recommend using the True-Nuclear™ Transcription Factor Buffer Set or the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor EGR1 staining.

RRID
AB_2941638 (BioLegend Cat. No. 943903)
AB_2941638 (BioLegend Cat. No. 943906)
AB_2941638 (BioLegend Cat. No. 943904)

Antigen Details

Structure
EGR1 is a 533 amino acid protein with a predicted molecular weight of 57 kD. The observed molecular weight ranges from 75 – 100 kD due to post-translational modifications.
Distribution

Ubiquitously expressed/ Nucleus and cytoplasm

Function
Transcription factor
Cell Type
Neurons
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. Cullen EM, et al. 2010. Mol Immunol. 47:1701-9.
  2. Duclot F & Kabbaj M. 2017. Front Behav Neurosci. 11:35.
  3. Havis E & Duprez D. 2020. Int J Mol Sci. 21:1664.
  4. Khachigian LM. 2006. Circ Res. 98:186-91.
  5. Li T, et al. 2019. Med Oncol. 37:7.
Gene ID
1958 View all products for this Gene ID
UniProt
View information about EGR1 on UniProt.org
Go To Top Version: 1    Revision Date: 04/13/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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